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Literature summary extracted from

  • Su, D.; Asard, H.
    Three mammalian cytochromes b561 are ascorbate-dependent ferrireductases (2006), FEBS J., 273, 3722-3734.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.1.3 L-galactono-gamma-lactone
-
Mus musculus
7.2.1.3 L-galactono-gamma-lactone
-
Homo sapiens
7.2.1.3 L-galactono-gamma-lactone
-
Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.3 expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity Mus musculus
7.2.1.3 expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity Homo sapiens
7.2.1.3 expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
7.2.1.3 D38A the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 E117A the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 E196A the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 F44A the mutation reduces the Fe(CN)3 reductase activity by about 45% Mus musculus
7.2.1.3 H105A the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 H112A the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 H117A the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme Mus musculus
7.2.1.3 H156A the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme Mus musculus
7.2.1.3 H47A the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme Mus musculus
7.2.1.3 H83A the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme Mus musculus
7.2.1.3 M51A the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 N106A the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 P48A the mutant shows increased reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 Q131A the activity of the mutant is reduced significantly to 45% of that of the wild type Mus musculus
7.2.1.3 R149A the mutation results in a 75% loss in activity compared to the wild type enzyme Mus musculus
7.2.1.3 R67A the mutation results in an almost complete loss of the Fe(CN)3 reductase activity Mus musculus
7.2.1.3 S115A the ferrireductase activity in the mutant is reduced to 50% Mus musculus
7.2.1.3 W119A the ferrireductase activity in the mutant is reduced to 17% Mus musculus
7.2.1.3 W119A the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 Y190A the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme Mus musculus
7.2.1.3 Y66A the mutation results in an almost complete loss of the Fe(CN)3 reductase activity Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.3 chromaffin granule
-
Mus musculus 42583
-
7.2.1.3 chromaffin granule
-
Homo sapiens 42583
-
7.2.1.3 chromaffin granule
-
Rattus norvegicus 42583
-
7.2.1.3 lysosome
-
Mus musculus 5764
-
7.2.1.3 lysosome
-
Homo sapiens 5764
-
7.2.1.3 lysosome
-
Rattus norvegicus 5764
-
7.2.1.3 plasma membrane
-
Mus musculus 5886
-
7.2.1.3 plasma membrane
-
Homo sapiens 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.1.3 27000
-
LCytb, SDS-PAGE Mus musculus
7.2.1.3 27800
-
CGCytb, SDS-PAGE Mus musculus
7.2.1.3 31500
-
DCytb, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.3 Homo sapiens
-
-
-
7.2.1.3 Mus musculus
-
-
-
7.2.1.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.1.3 duodenum
-
Mus musculus
-
7.2.1.3 duodenum
-
Homo sapiens
-
7.2.1.3 duodenum
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.3 ascorbate[side 1] + Fe(CN)3[side 2]
-
Mus musculus monodehydroascorbate[side 1] + ?
-
?
7.2.1.3 ascorbate[side 1] + Fe(CN)3[side 2]
-
Homo sapiens monodehydroascorbate[side 1] + ?
-
?
7.2.1.3 ascorbate[side 1] + Fe(CN)3[side 2]
-
Rattus norvegicus monodehydroascorbate[side 1] + ?
-
?
7.2.1.3 ascorbate[side 1] + Fe3+-EDTA[side 2]
-
Mus musculus monodehydroascorbate[side 1] + ?
-
?
7.2.1.3 ascorbate[side 1] + Fe3+-EDTA[side 2]
-
Homo sapiens monodehydroascorbate[side 1] + ?
-
?
7.2.1.3 ascorbate[side 1] + Fe3+-EDTA[side 2]
-
Rattus norvegicus monodehydroascorbate[side 1] + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.3 ascorbate-dependent ferrireductase
-
Mus musculus
7.2.1.3 ascorbate-dependent ferrireductase
-
Homo sapiens
7.2.1.3 ascorbate-dependent ferrireductase
-
Rattus norvegicus
7.2.1.3 CGCytb
-
Mus musculus
7.2.1.3 CGCytb
-
Homo sapiens
7.2.1.3 CGCytb
-
Rattus norvegicus
7.2.1.3 chromaffin granule cytochrome b
-
Mus musculus
7.2.1.3 chromaffin granule cytochrome b
-
Homo sapiens
7.2.1.3 chromaffin granule cytochrome b
-
Rattus norvegicus
7.2.1.3 Cyt b561
-
Mus musculus
7.2.1.3 Cyt b561
-
Homo sapiens
7.2.1.3 Cyt b561
-
Rattus norvegicus
7.2.1.3 DCytb
-
Mus musculus
7.2.1.3 DCytb
-
Homo sapiens
7.2.1.3 duodenal cytochrome b
-
Mus musculus
7.2.1.3 duodenal cytochrome b
-
Homo sapiens
7.2.1.3 LCytb
-
Mus musculus
7.2.1.3 LCytb
-
Homo sapiens
7.2.1.3 LCytb
-
Rattus norvegicus
7.2.1.3 lysosomal cytochrome b
-
Mus musculus
7.2.1.3 lysosomal cytochrome b
-
Homo sapiens
7.2.1.3 lysosomal cytochrome b
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.3 ascorbate dependent on Mus musculus
7.2.1.3 ascorbate dependent on Homo sapiens
7.2.1.3 ascorbate dependent on Rattus norvegicus