Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bernard, L.; Desplats, C.; Mus, F.; Cuine, S.; Cournac, L.; Peltier, G.
    Agrobacterium tumefaciens type II NADH dehydrogenase. Characterization and interactions with bacterial and thylakoid membranes (2006), FEBS J., 273, 3625-3637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2 expressed in Escherichia coli Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 diphenylene iodonium 75% inhibition, IC50: 0.013 mM Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.005
-
 NADH in the presence of 1 mM ferricyanide Agrobacterium tumefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.2 46000
-
 1 * 46000, SDS-PAGE Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 nickel-affinity chromatography Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 2 ferricyanide + NADH
-
 Agrobacterium tumefaciens 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 decyl-ubiquinone + NADH
-
 Agrobacterium tumefaciens decyl-ubiquinol + NAD+
-
 ?
7.1.1.2 NADH + plastoquinone
-
 Agrobacterium tumefaciens NAD+ + plastoquinol
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Agrobacterium tumefaciens NAD+ + ubiquinol
-
 ?
7.1.1.2 NADPH + H+ + ubiquinone the enzyme has a lower affinity to NADPH than to NADH Agrobacterium tumefaciens NADP+ + ubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 monomer 1 * 46000, SDS-PAGE Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NDH-2
-
 Agrobacterium tumefaciens
7.1.1.2 type II NADH dehydrogenase
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FAD
-
 Agrobacterium tumefaciens
7.1.1.2 NADH
-
 Agrobacterium tumefaciens
7.1.1.2 NADPH much higher affinity for NADH than for NADPH Agrobacterium tumefaciens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.1.1.2 0.013
-
 75% inhibition, IC50: 0.013 mM Agrobacterium tumefaciens diphenylene iodonium