EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | oxygen | seems to promote very low DHODH activity | Candida albicans |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.5.2 | medicine | background for the development of antipyrimidines with high efficacy for decreasing in situ pyrimidine nucleotide pools in Candida albicans | Candida albicans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.2 | full-length DHODH and N-terminally truncated DHODH, which lacks the targeting sequence and the transmembrane domain recombinantly expressed in Escherichia coli BL21 | Candida albicans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.2 | L11S | two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system | Candida albicans |
1.3.5.2 | L78S | two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system | Candida albicans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | brequinar sodium | 1 mM reduces the activity by more than 50% of the full-length enzyme | Candida albicans | |
1.3.5.2 | redoxal | 0.5 mM exhibit an inhibitory effect of more than 50% on full-length DHODH and N-terminally truncated DHODH compared with the noninhibited reaction | Candida albicans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 0.04 | - |
dihydroorotate | N-terminally truncated DHODH, fixed concentration of dihydroorotate and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM | Candida albicans | |
1.3.5.2 | 0.042 | - |
dihydroorotate | full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM | Candida albicans | |
1.3.5.2 | 0.065 | - |
dihydroorotate | N-terminally truncated DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM | Candida albicans | |
1.3.5.2 | 0.108 | - |
dihydroorotate | full-length DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol | Candida albicans | |
1.3.5.2 | 0.111 | - |
dihydroorotate | N-terminally truncated DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol | Candida albicans | |
1.3.5.2 | 0.122 | - |
dihydroorotate | full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM | Candida albicans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | 48000 | - |
48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE | Candida albicans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.2 | Candida albicans | Q874I4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.5.2 | full-length DHODH and N-terminally truncated DHODH, by affinity chromatography | Candida albicans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | 6 | - |
using decylubiquinone and 2,6-dichloroindophenol as acceptor | Candida albicans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.2 | dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Candida albicans | orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
? | |
1.3.5.2 | dihydroorotate + 2,6-dichloroindophenol | - |
Candida albicans | orotate + reduced 2,6-dichloroindophenol | - |
? | |
1.3.5.2 | dihydroorotate + decylubiquinone | - |
Candida albicans | orotate + reduced decylubiquinone | - |
? | |
1.3.5.2 | dihydroorotate + potassium hexacyanoferrate(III) | - |
Candida albicans | orotate + reduced potassium hexacyanoferrate(III) | - |
? | |
1.3.5.2 | dihydroorotate + ubiquinone 30 | - |
Candida albicans | orotate + reduced ubiquinone 30 | - |
? | |
1.3.5.2 | dihydroorotate + ubiquinone 50 | - |
Candida albicans | orotate + reduced ubiquinone 50 | - |
? | |
1.3.5.2 | additional information | fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH | Candida albicans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.5.2 | ? | 48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE | Candida albicans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.2 | DHODH | - |
Candida albicans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 2.1 | - |
dihydroorotate | full-length DHODH | Candida albicans | |
1.3.5.2 | 2.2 | - |
dihydroorotate | N-terminally truncated DHODH | Candida albicans | |
1.3.99.11 | 2.1 | - |
L-dihydroorotate | - |
Candida albicans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | additional information | - |
8.0-8.5 | Candida albicans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | flavin | - |
Candida albicans |