Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kawakami, R.; Sakuraba, H.; Tsuge, H.; Goda, S.; Katunuma, N.; Ohshima, T.
    A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 (2005), FEBS J., 272, 4044-4054.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 0.03
-
 2,6-dichlorophenolindophenol isoform PDH1 Pyrococcus horikoshii
1.5.99.B2 4
-
 L-proline isoform PDH1 Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 8000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 20000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 43000
-
 isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 46000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 52000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 56000
-
 isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 101000
-
 isoform PDH2, gel filtration Pyrococcus horikoshii
1.5.99.B2 440000
-
 isoform PDH1, gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pyrococcus horikoshii
-
 isoforms PDH1 and PDH2
-
1.5.99.B2 Pyrococcus horikoshii OT-3
-
 isoforms PDH1 and PDH2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.B2 0.939
-
 isoform PDH1 Pyrococcus horikoshii
1.5.99.B2 1.4
-
 isoform PDH2 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Pyrococcus horikoshii (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Pyrococcus horikoshii OT-3 (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 additional information isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid Pyrococcus horikoshii ?
-
 ?
1.5.99.B2 additional information isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.B2 heterotetramer isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 octamer isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.99.B2 90
-
 isoform PDH1, stable for at least 120 min Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.B2 7.5
-
 isoform PDH1 Pyrococcus horikoshii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.99.B2 5 10 isoform PDH1, 50°C, stable for at least 30 min Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 ATP isoform PDH1, 1 mol per mol of alpha subunit Pyrococcus horikoshii
1.5.99.B2 FAD isoform PDH1, 1 mol per mol of beta subunit, alpha and beta subunits separatively produced in Escherichia coli cannot bind FAD Pyrococcus horikoshii
1.5.99.B2 FMN situated at the interface of alpha and beta subunit Pyrococcus horikoshii