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Literature summary extracted from

  • Liu, B.; Hong, Y.; Wu, L.; Li, Z.; Ni, J.; Sheng, D.; Shen, Y.
    A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic archaeon Pyrococcus horikoshii: gene cloning, expression and characterization (2007), Extremophiles, 11, 733-739.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.165 additional information 50 mM NaN3, 10 mM phenylmethyl sulfonylfluoride, and H2O2 have no obvious effect on the enzymatic activity Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.165
-
Pyrococcus horikoshii
2.7.1.165 expressed in Escherichia coli Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 CuCl2 1 mM, 61% inhibition; 61% inhibition at 1 mM Pyrococcus horikoshii
2.7.1.165 HgCl2 11% inhibition at 1 mM; 1 mM, 11% inhibition Pyrococcus horikoshii
2.7.1.165 additional information resistant to the urea, ethanol, 2-propanol, n-butanol and DMSO (10% v/v each) Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.044
-
D-glycerate
-
Pyrococcus horikoshii
2.7.1.165 0.044
-
(R)-glycerate the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C Pyrococcus horikoshii
2.7.1.165 0.102
-
ATP
-
Pyrococcus horikoshii
2.7.1.165 0.102
-
ATP the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Co2+ 10 mM, 68% of the activity with Mg2+ Pyrococcus horikoshii
2.7.1.165 K+ 7.94fold increase of activity at 50 mM Pyrococcus horikoshii
2.7.1.165 K+ 50 mM, 7.9fold activation Pyrococcus horikoshii
2.7.1.165 Li+ 2.28fold increase of activity at 50 mM Pyrococcus horikoshii
2.7.1.165 Li+ 50 mM, 2.3fold activation Pyrococcus horikoshii
2.7.1.165 Mg2+ 10 mM, required for activity Pyrococcus horikoshii
2.7.1.165 Mg2+ no activity is observed in the absence of divalent metal ion and maximal activity is observed in the presence of Mg2+ (10 mM) Pyrococcus horikoshii
2.7.1.165 Mn2+ 10 mM, 76% of the activity with Mg2+ Pyrococcus horikoshii
2.7.1.165 additional information when Mn2+, Co2+, Ca2+, Sr2+ and Ni2+ is substituted for Mg2+, respectively, Mn2+, Co2+ and Ni2+ show 76, 68 and 11% activity of that for Mg2+ Pyrococcus horikoshii
2.7.1.165 Na+ 3.49fold increase of activity at 50 mM Pyrococcus horikoshii
2.7.1.165 Na+ 50 mM, 3.5fold activation Pyrococcus horikoshii
2.7.1.165 NH4+ 7.83fold increase of activity at 50 mM Pyrococcus horikoshii
2.7.1.165 NH4+ 50 mM, 7.8fold activation Pyrococcus horikoshii
2.7.1.165 Ni2+ 10 mM, 11% of the activity with Mg2+ Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 47000
-
SDS-PAGE Pyrococcus horikoshii
2.7.1.165 47000
-
2 * 47000, SDS-PAGE Pyrococcus horikoshii
2.7.1.165 47400
-
calculated from amino acid sequence Pyrococcus horikoshii
2.7.1.165 50000
-
2 * 50000, gel filtration Pyrococcus horikoshii
2.7.1.165 100000
-
gel filtration Pyrococcus horikoshii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.1.165 2-propanol resistant to Pyrococcus horikoshii
2.7.1.165 DMSO resistant to Pyrococcus horikoshii
2.7.1.165 Ethanol resistant to Pyrococcus horikoshii
2.7.1.165 n-Butanol resistant to Pyrococcus horikoshii
2.7.1.165 urea resistant to Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Pyrococcus horikoshii
-
-
-
2.7.1.165 Pyrococcus horikoshii O58231
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165 nickel affinity chromatography, HiTrap Q ion exchange chromatography, and SephacrylTM S-200 HR gel filtration Pyrococcus horikoshii

Storage Stability

EC Number Storage Stability Organism
2.7.1.165 90°C, 50 mM Tris-HCl and 100 mM NaCl buffer (pH 8.0), 12 h, almost no loss of activity Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ADP + (R)-glycerate at 32% of the activity with ATP Pyrococcus horikoshii AMP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 ADP + (R)-glycerate 32% of the activity with ATP Pyrococcus horikoshii AMP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate
-
Pyrococcus horikoshii ADP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate
-
Pyrococcus horikoshii ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + D-glycerate
-
Pyrococcus horikoshii ADP + 2-phospho-D-glycerate
-
?
2.7.1.165 CTP + (R)-glycerate at 73% of the activity with ATP Pyrococcus horikoshii CDP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 CTP + (R)-glycerate 73% of the activity with ATP Pyrococcus horikoshii CDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 diphosphate + (R)-glycerate at 112% of the activity with ATP Pyrococcus horikoshii phosphate + 3-phospho-(R)-glycerate
-
?
2.7.1.165 diphosphate + (R)-glycerate 112% of the activity with ATP Pyrococcus horikoshii phosphate + 2-phospho-(R)-glycerate
-
?
2.7.1.165 GTP + (R)-glycerate at 64% of the activity with ATP Pyrococcus horikoshii GDP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 GTP + (R)-glycerate 64% of the activity with ATP Pyrococcus horikoshii GDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 additional information AMP is not a substrate Pyrococcus horikoshii ?
-
?
2.7.1.165 UTP + (R)-glycerate at 29% of the activity with ATP Pyrococcus horikoshii UDP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 UTP + (R)-glycerate 29% of the activity with ATP Pyrococcus horikoshii UDP + 2-phospho-(R)-glycerate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 dimer 2 * 47000, SDS-PAGE Pyrococcus horikoshii
2.7.1.165 dimer 2 * 50000, gel filtration Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.165 2-PGA forming glycerate kinase
-
Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 45
-
-
Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.165 30 50 strong activity at 30-50°C Pyrococcus horikoshii
2.7.1.165 45 100 45°C: optimum, 100°C: about 50% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.165 90
-
12 h, no loss of activity Pyrococcus horikoshii
2.7.1.165 100
-
about half of the maximal activity remains at 100°C Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.165 360
-
(R)-glycerate the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C Pyrococcus horikoshii
2.7.1.165 489.2
-
D-glycerate
-
Pyrococcus horikoshii
2.7.1.165 500.6
-
ATP
-
Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.165 7
-
-
Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.165 6 10 50% of maximal activity at pH 6.0 and 10.0 Pyrococcus horikoshii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.165 6 10 half of the maximum activity remains at pH 6-10 Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.165 ATP
-
Pyrococcus horikoshii