BRENDA - Enzyme Database show

Arsenic (+3 oxidation state) methyltransferase and the methylation of arsenicals

Thomas, D.J.; Li, J.; Waters, S.B.; Xing, W.; Adair, B.M.; Drobna, Z.; Devesa, V.; Styblo, M.; Exp. Biol. Med. 232, 3-13 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Homo sapiens
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Rattus norvegicus
2.1.1.137
thioredoxin
dependent on, activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic
Homo sapiens
2.1.1.137
thioredoxin
activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expression of rat AS3MT in a simian virus 40 (SV40)-transformed human urothelial cell line confers the capacity to methylate inorganic arsenic on cells that otherwise do not express AS3MT and that have a null phenotype for iAs methylation; gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Rattus norvegicus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Bos taurus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Ciona intestinalis
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Gallus gallus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Mus musculus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Oncorhynchus mykiss
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Pan troglodytes
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Rhodopseudomonas palustris
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Strongylocentrotus purpuratus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT, recombinant expression
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Homo sapiens
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Homo sapiens
5829
-
2.1.1.137
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
42000
-
x * 42000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
additional information
Gallus gallus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Mus musculus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Rhodopseudomonas palustris
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Oncorhynchus mykiss
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Strongylocentrotus purpuratus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Ciona intestinalis
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Pan troglodytes
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Rattus norvegicus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Homo sapiens
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Bos taurus
enzyme evolutionary analysis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Bos taurus
-
-
-
2.1.1.137
Bos taurus
Q58DQ0
gene arsM
-
2.1.1.137
Ciona intestinalis
-
; gene arsM
-
2.1.1.137
Gallus gallus
-
; gene arsM
-
2.1.1.137
Homo sapiens
Q9HBK9
; gene arsM
-
2.1.1.137
Mus musculus
-
; gene arsM
-
2.1.1.137
no activity in Caenorhabditis elegans
-
-
-
2.1.1.137
no activity in Drosophila melanogaster
-
-
-
2.1.1.137
Oncorhynchus mykiss
-
; gene arsM
-
2.1.1.137
Pan troglodytes
-
; gene arsM
-
2.1.1.137
Rattus norvegicus
Q8VHT6
adult male Fischer 344 rats, gene arsM
-
2.1.1.137
Rattus norvegicus
-
-
-
2.1.1.137
Rhodopseudomonas palustris
-
gene arsM
-
2.1.1.137
Strongylocentrotus purpuratus
-
gene arsM
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
native enzyme several thousandfold using pH-dependent fractionation, chromatofocusing, and S-adenosylhomocysteine-affinity chromatography, the purification of AS3MT from liver cytosol is dependent on the presence of 1 mM dithiothreitol and 5 mM GSH
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Bos taurus
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Ciona intestinalis
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Gallus gallus
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Homo sapiens
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Mus musculus
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Oncorhynchus mykiss
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Pan troglodytes
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Rattus norvegicus
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Rhodopseudomonas palustris
2.1.1.137
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
reaction modelling, overview
Strongylocentrotus purpuratus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
hepatocyte
primary
Homo sapiens
-
2.1.1.137
liver
-
Homo sapiens
-
2.1.1.137
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Gallus gallus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Mus musculus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Rhodopseudomonas palustris
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Oncorhynchus mykiss
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Strongylocentrotus purpuratus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Ciona intestinalis
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Pan troglodytes
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Rattus norvegicus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Homo sapiens
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Bos taurus
?
-
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Gallus gallus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Mus musculus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Rattus norvegicus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Bos taurus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Oncorhynchus mykiss
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Ciona intestinalis
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Pan troglodytes
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 42000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.137
37
-
assay at
Homo sapiens
2.1.1.137
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7
7.4
assay at
Homo sapiens
2.1.1.137
7
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Bos taurus
2.1.1.137
S-adenosyl-L-methionine
-
Ciona intestinalis
2.1.1.137
S-adenosyl-L-methionine
-
Gallus gallus
2.1.1.137
S-adenosyl-L-methionine
-
Oncorhynchus mykiss
2.1.1.137
S-adenosyl-L-methionine
-
Pan troglodytes
2.1.1.137
S-adenosyl-L-methionine
-
Rattus norvegicus
2.1.1.137
S-adenosyl-L-methionine
-
Strongylocentrotus purpuratus
2.1.1.137
S-adenosyl-L-methionine
-
Mus musculus
2.1.1.137
S-adenosyl-L-methionine
-
Rhodopseudomonas palustris
2.1.1.137
S-adenosyl-L-methionine
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Homo sapiens
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Rattus norvegicus
2.1.1.137
thioredoxin
dependent on, activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic
Homo sapiens
2.1.1.137
thioredoxin
activates formation of methyl arsenic, dimethyl arsenic, and trimethyl arsenic
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expression of rat AS3MT in a simian virus 40 (SV40)-transformed human urothelial cell line confers the capacity to methylate inorganic arsenic on cells that otherwise do not express AS3MT and that have a null phenotype for iAs methylation
Rattus norvegicus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Bos taurus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Ciona intestinalis
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Gallus gallus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Mus musculus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Oncorhynchus mykiss
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Pan troglodytes
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Rattus norvegicus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Rhodopseudomonas palustris
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
Strongylocentrotus purpuratus
2.1.1.137
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT, recombinant expression
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Bos taurus
2.1.1.137
S-adenosyl-L-methionine
-
Ciona intestinalis
2.1.1.137
S-adenosyl-L-methionine
-
Gallus gallus
2.1.1.137
S-adenosyl-L-methionine
-
Mus musculus
2.1.1.137
S-adenosyl-L-methionine
-
Oncorhynchus mykiss
2.1.1.137
S-adenosyl-L-methionine
-
Pan troglodytes
2.1.1.137
S-adenosyl-L-methionine
-
Rattus norvegicus
2.1.1.137
S-adenosyl-L-methionine
-
Strongylocentrotus purpuratus
2.1.1.137
S-adenosyl-L-methionine
-
Rhodopseudomonas palustris
2.1.1.137
S-adenosyl-L-methionine
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Homo sapiens
2.1.1.137
GSH
addition of GSH stimulates the overall rate for As methylation, but inhibits formation of trimethylarsenite
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Homo sapiens
5829
-
2.1.1.137
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
42000
-
x * 42000, SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
additional information
Gallus gallus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Mus musculus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Rhodopseudomonas palustris
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Oncorhynchus mykiss
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Strongylocentrotus purpuratus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Ciona intestinalis
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Pan troglodytes
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Rattus norvegicus
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Homo sapiens
enzyme evolutionary analysis
?
-
-
-
2.1.1.137
additional information
Bos taurus
enzyme evolutionary analysis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
native enzyme several thousandfold using pH-dependent fractionation, chromatofocusing, and S-adenosylhomocysteine-affinity chromatography, the purification of AS3MT from liver cytosol is dependent on the presence of 1 mM dithiothreitol and 5 mM GSH
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
hepatocyte
primary
Homo sapiens
-
2.1.1.137
liver
-
Homo sapiens
-
2.1.1.137
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Gallus gallus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Mus musculus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Rhodopseudomonas palustris
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Oncorhynchus mykiss
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Strongylocentrotus purpuratus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Ciona intestinalis
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Pan troglodytes
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Rattus norvegicus
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Homo sapiens
?
-
-
-
-
2.1.1.137
additional information
enzyme evolutionary analysis
673463
Bos taurus
?
-
-
-
-
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Gallus gallus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Mus musculus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Rattus norvegicus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Bos taurus
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Oncorhynchus mykiss
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Ciona intestinalis
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Pan troglodytes
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
673463
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 42000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.137
37
-
assay at
Homo sapiens
2.1.1.137
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7
7.4
assay at
Homo sapiens
2.1.1.137
7
-
assay at
Rattus norvegicus