Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ferrer-Sevillano, F.; Fernandez-Canon, J.M.
    Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities (2007), Eukaryot. Cell, 6, 514-520.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.63 additional information phenylacetate and its hydroxyderivatives strongly induce the enzyme Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.63 DNA and amino acid sequence determination and analysis, expression pattern, sequence comparison, expression in a recombinant Aspergillus nidulans strain Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.63 additional information a phacB-disrupted strain DELTAphacB does not grow on 3-hydroxy-, 4-hydroxy-, or 3,4-dihydroxy-phenylacetate, overview Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.63 Fe2+ the enzyme is a cytochrome P450 monooxygenase containing a heme iron Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans BiA1 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans BiA1 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
1.14.13.63 3-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.63 Aspergillus nidulans Q078T0 gene phacB
-
1.14.13.63 Aspergillus nidulans BiA1 Q078T0 gene phacB
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.63 mycelium
-
 Aspergillus nidulans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 low activity Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 low activity Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 2-hydroxyphenylacetate + NAD(P)H + O2 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2
-
 Aspergillus nidulans 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2
-
 Aspergillus nidulans BiA1 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans BiA1 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
1.14.13.63 3-hydroxyphenylacetate + NAD(P)H + O2
-
 Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.13.63 3-hydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
1.14.13.63 3-hydroxyphenylacetate + NAD(P)H + O2
-
 Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.63 3,4-dihydroxyphenylacetate 6-hydroxylase
-
 Aspergillus nidulans
1.14.13.63 CYP504B
-
 Aspergillus nidulans
1.14.13.63 More the enzyme is a member of a the CYP450 subfamily Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.63 37
-
 assay at Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.63 7
-
 assay at Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.63 NADH
-
 Aspergillus nidulans
1.14.13.63 NADPH
-
 Aspergillus nidulans