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Literature summary extracted from

  • Mukherjee, M.; Sievers, S.A.; Brown, M.T.; Johnson, P.J.
    Identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of Trichomonas vaginalis (2006), Eukaryot. Cell, 5, 2072-2078.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.1 pyridoxal phosphate
-
 Trichomonas vaginalis

Application

EC Number Application Comment Organism
2.1.2.1 medicine enzyme is distinct from its prokaryotic and eukaryotic counterparts, thus is a potential drug target Trichomonas vaginalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 wild-type and mutants cloned into vector pET-29B and expressed in Escherichia coli BL21(DE3) cells, moreover expressed in Trichomonas vaginalis with a C-terminal HA tag Trichomonas vaginalis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 K251Q oligomeric structure not affected, but inactive in both the presence and absence of pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 K251R oligomeric structure not affected, catalytic activity in the presence of pyridoxal phosphate also unaffected Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.3
-
 tetrahydrofolate mutant K251R, without pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 0.89
-
 tetrahydrofolate wild-type, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 1
-
 tetrahydrofolate wild-type, without pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 1.16
-
 tetrahydrofolate mutant K251R, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 hydrogenosome enzyme possesses a putative N-terminal hydrogenosomal presequence Trichomonas vaginalis 42566
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 50000
-
 sequence analysis Trichomonas vaginalis

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Trichomonas vaginalis
-
 strains T1 and G3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 by Ni affinity chromatography, more than 98% pure Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Trichomonas vaginalis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homodimer immunoblotting Trichomonas vaginalis

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyl transferase
-
 Trichomonas vaginalis
2.1.2.1 SHMT
-
 Trichomonas vaginalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.005
-
 tetrahydrofolate mutant K251R, without pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 1.13
-
 tetrahydrofolate mutant K251R, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 1.29
-
 tetrahydrofolate wild-type, without pyridoxal phosphate Trichomonas vaginalis
2.1.2.1 1.3
-
 tetrahydrofolate wild-type, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis