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Literature summary extracted from

  • Morris, M.T.; DeBruin, C.; Yang, Z.; Chambers, J.W.; Smith, K.S.; Morris, J.C.
    Activity of a second Trypanosoma brucei hexokinase is controlled by an 18-amino-acid C-terminal tail (2006), Eukaryot. Cell, 5, 2014-2023.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Escherichia coli, recombinant TbHK2 lacks detectable enzymatic activity. Replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1 Trypanosoma brucei
2.7.1.1 expression in Escherichia coli, TbHK1 Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.1 additional information replacing the last 18 amino acids of TbHK1 with the corresponding residues of TbHK2 yields an inactive recombinant protein Trypanosoma brucei
2.7.1.1 additional information replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1 Trypanosoma brucei
2.7.1.1 N469D inactive mutant protein, TbHK1 Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 lauric acid IC50: 0.0758 mM, TbHK1 Trypanosoma brucei
2.7.1.1 myristic acid IC50: 0.0784 mM, TbHK1 Trypanosoma brucei
2.7.1.1 palmitic acid IC50: 0.0624 mM, TbHK1 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.009
-
 D-glucose recombinant TbHK1 Trypanosoma brucei
2.7.1.1 0.03
-
 D-mannose recombinant TbHK1 Trypanosoma brucei
2.7.1.1 0.035
-
 D-fructose recombinant TbHK1 Trypanosoma brucei
2.7.1.1 0.28
-
 ATP recombinant TbHK1 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Trypanosoma brucei Q95PL2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Trypanosoma brucei ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Trypanosoma brucei ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP
-
 Trypanosoma brucei ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP
-
 Trypanosoma brucei ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP
-
 Trypanosoma brucei ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 additional information the C-terminal tail of TbHK1 is important for hexokinase activity Trypanosoma brucei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 TbHK1
-
 Trypanosoma brucei
2.7.1.1 TbHK2
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.1 483.3
-
 D-glucose recombinant TbHK1 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.1 0.0624
-
 IC50: 0.0624 mM, TbHK1 Trypanosoma brucei palmitic acid
2.7.1.1 0.0758
-
 IC50: 0.0758 mM, TbHK1 Trypanosoma brucei lauric acid
2.7.1.1 0.0784
-
 IC50: 0.0784 mM, TbHK1 Trypanosoma brucei myristic acid