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Literature summary extracted from

  • Rosconi, F.; Fraguas, L.F.; Martinez-Drets, G.; Castro-Sowinski, S.
    Purification and characterization of a periplasmic laccase produced by Sinorhizobium meliloti (2005), Enzyme Microb. Technol., 36, 800-807.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.10.3.2 ammonium sulfate stabilizes Sinorhizobium meliloti
1.10.3.2 no satnilization by EDTA and Cu2+ Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol
-
Sinorhizobium meliloti
1.10.3.2 Cu2+
-
Sinorhizobium meliloti
1.10.3.2 Fe3+
-
Sinorhizobium meliloti
1.10.3.2 Mn2+
-
Sinorhizobium meliloti
1.10.3.2 NaCl
-
Sinorhizobium meliloti
1.10.3.2 NaCN
-
Sinorhizobium meliloti
1.10.3.2 NaF
-
Sinorhizobium meliloti
1.10.3.2 reduced glutathione
-
Sinorhizobium meliloti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 periplasm
-
Sinorhizobium meliloti
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 (NH4)2SO4 4fold activation Sinorhizobium meliloti
1.10.3.2 imidazole about 2fold activation Sinorhizobium meliloti
1.10.3.2 K2SO4 3.2fold activation Sinorhizobium meliloti
1.10.3.2 Na2SO4 3.5fold activation Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 45000
-
2 * 45000, SDS-PAGE Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Sinorhizobium meliloti
-
-
-
1.10.3.2 Sinorhizobium meliloti CE52G
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 29fold by a two-step procedure involving heat treatment and immobilized metal affinity chromatography Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 0.402
-
purified enzyme Sinorhizobium meliloti

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 45000, SDS-PAGE Sinorhizobium meliloti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 70
-
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti
1.10.3.2 75
-
purified enzyme, inactivation Sinorhizobium meliloti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4 8 the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 Sinorhizobium meliloti

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Sinorhizobium meliloti isoelectric focusing
-
6.2