BRENDA - Enzyme Database

Inhibition of laccase activity from Trametes versicolor by heavy metals and organic compounds

Lorenzo, M.; Moldes, D.; Rodriguez Couto, S.; Sanroman, M.A.; Chemosphere 60, 1124-1128 (2005)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.2
Cd2+
40% inhibition at 20 mM, almost complete inhibition at 80 mM
Trametes versicolor
1.10.3.2
Citric acid
-
Trametes versicolor
1.10.3.2
Cu2+
40% inhibition at 20 mM, almost complete inhibition at 80 mM
Trametes versicolor
1.10.3.2
EDTA
slight inhibition
Trametes versicolor
1.10.3.2
hydroxylammonium chloride
-
Trametes versicolor
1.10.3.2
malonic acid
-
Trametes versicolor
1.10.3.2
Mn2+
-
Trametes versicolor
1.10.3.2
oxalic acid
-
Trametes versicolor
1.10.3.2
sulfamic acid
-
Trametes versicolor
1.10.3.2
Zn2+
-
Trametes versicolor
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.2
0.008
-
syringaldazine
pH 4.5, 25°C
Trametes versicolor
1.10.3.2
0.026
-
2,6-dimethoxyphenol
pH 4.5, 25°C
Trametes versicolor
1.10.3.2
0.038
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5, 25°C
Trametes versicolor
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.10.3.2
Cu2+
opper-containing enzyme
Trametes versicolor
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.2
Trametes versicolor
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
673096
Trametes versicolor
?
-
-
-
?
1.10.3.2
2,6-dimethoxyphenol + O2
-
673096
Trametes versicolor
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
-
-
?
1.10.3.2
syringaldazine + O2
prefrred substrate
673096
Trametes versicolor
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.2
25
-
assay at
Trametes versicolor
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
4.5
-
assay at
Trametes versicolor
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.2
Cd2+
40% inhibition at 20 mM, almost complete inhibition at 80 mM
Trametes versicolor
1.10.3.2
Citric acid
-
Trametes versicolor
1.10.3.2
Cu2+
40% inhibition at 20 mM, almost complete inhibition at 80 mM
Trametes versicolor
1.10.3.2
EDTA
slight inhibition
Trametes versicolor
1.10.3.2
hydroxylammonium chloride
-
Trametes versicolor
1.10.3.2
malonic acid
-
Trametes versicolor
1.10.3.2
Mn2+
-
Trametes versicolor
1.10.3.2
oxalic acid
-
Trametes versicolor
1.10.3.2
sulfamic acid
-
Trametes versicolor
1.10.3.2
Zn2+
-
Trametes versicolor
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.2
0.008
-
syringaldazine
pH 4.5, 25°C
Trametes versicolor
1.10.3.2
0.026
-
2,6-dimethoxyphenol
pH 4.5, 25°C
Trametes versicolor
1.10.3.2
0.038
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5, 25°C
Trametes versicolor
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.10.3.2
Cu2+
opper-containing enzyme
Trametes versicolor
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
673096
Trametes versicolor
?
-
-
-
?
1.10.3.2
2,6-dimethoxyphenol + O2
-
673096
Trametes versicolor
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
-
-
?
1.10.3.2
syringaldazine + O2
prefrred substrate
673096
Trametes versicolor
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.2
25
-
assay at
Trametes versicolor
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
4.5
-
assay at
Trametes versicolor