Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duckworth, B.P.; Zhang, Z.; Hosokawa, A.; Distefano, M.D.
    Selective labeling of proteins by using protein farnesyltransferase (2007), ChemBioChem, 8, 98-105.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.58 0.00042
-
 farnesyl diphosphate in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, and 20 nM enzyme, at 30°C Saccharomyces cerevisiae
2.5.1.58 0.006
-
 N-dansyl-GCVIA in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, 20 nM enzyme, and 5 mM DTT, at 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.58 Mg2+ 10 mM, required Saccharomyces cerevisiae
2.5.1.58 Zn2+ 0.01 mM, required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine Saccharomyces cerevisiae
-
 diphosphate + S-farnesyl protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.58 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine
-
 Saccharomyces cerevisiae diphosphate + S-farnesyl protein
-
 ?
2.5.1.58 geranyl alkyne diphosphate + N-dansyl-GCVIA
-
 Saccharomyces cerevisiae N-dansyl-G-(alkyne geranyl)CVIA + diphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.5.1.58 PFTase
-
 Saccharomyces cerevisiae