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Literature summary extracted from

  • Steffan, N.; Unsoeld, I.A.; Li, S.M.
    Chemoenzymatic synthesis of prenylated indole derivatives by using a 4-dimethylallyltryptophan synthase from Aspergillus fumigatus (2007), ChemBioChem, 8, 1298-1307.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 expressed in Escherichia coli BL21(DE3) pLysS cells Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.009
-
L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.039
-
5-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.103
-
6-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.129
-
7-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.13
-
N-methyl-L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.186
-
L-tryptophan methyl ester in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.242
-
L-beta-homotryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.35
-
alpha-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.509
-
5-hydroxy-L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.558
-
Indole-3-propionic acid in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 57000
-
SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34 Ni-NTA agarose chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-5-hydroxy-L-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + alpha-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-alpha-methyl-DL-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + D-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-D-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + DL-indole-3-lactic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-propionic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-pyruvic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid
-
?
2.5.1.34 dimethylallyl diphosphate + L-beta-homotryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-homotryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan hydroxamate
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan hydroxamate
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan methyl ester
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester
-
?
2.5.1.34 dimethylallyl diphosphate + N-methyl-L-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-N-methyl-L-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + serotonin
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-serotonin
-
?
2.5.1.34 dimethylallyl diphosphate + tryptamin
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-tryptamin
-
?
2.5.1.34 diphosphate + 7-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-DL-tryptophan
-
?
2.5.1.34 additional information halogenated derivatives of tryptophan are not accepted as substrates Aspergillus fumigatus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 4-dimethylallyltryptophan synthase
-
Aspergillus fumigatus
2.5.1.34 FgaPT2
-
Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.34 0.0533
-
6-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.0683
-
5-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.1033
-
alpha-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.1267
-
7-methyl-DL-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.1367
-
L-tryptophan methyl ester in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.165
-
5-hydroxy-L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.252
-
N-methyl-L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.2667
-
L-tryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.2683
-
Indole-3-propionic acid in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus
2.5.1.34 0.3767
-
L-beta-homotryptophan in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 Aspergillus fumigatus