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Literature summary extracted from

  • Wu, Y.; Waldmann, H.; Reents, R.; Ebetino, F.H.; Goody, R.S.; Alexandrov, K.
    A protein fluorescence amplifier: continuous fluorometric assay for Rab geranylgeranyltransferase (2006), Chembiochem, 7, 1859-1861.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.60 NE10790 a weak bisphosphonate inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.60 additional information
-
additional information kinetics Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.60 geranylgeranyl diphosphate + Rab protein Rattus norvegicus
-
S-geranylgeranyl-Rab protein + diphosphate
-
?
2.5.1.60 additional information Rattus norvegicus RabGGTase, which prenylates more than 60 members of the Rab GTPase family, can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.60 Rattus norvegicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.60 additional information
-
development of a continuous fluorometric assay using a protein fluorescence amplifier, overview Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.60 geranylgeranyl diphosphate + Rab protein
-
Rattus norvegicus S-geranylgeranyl-Rab protein + diphosphate
-
?
2.5.1.60 geranylgeranyl diphosphate + Rab7 protein the N-terminal Cys207 is preferred to Cys205, activity with Rab7 mutants, overview Rattus norvegicus S-geranylgeranyl-Rab7 protein + diphosphate
-
?
2.5.1.60 additional information RabGGTase, which prenylates more than 60 members of the Rab GTPase family, can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein Rattus norvegicus ?
-
?
2.5.1.60 [3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)dodeca-2,6,10-trien-1] diphosphate + Rab7 protein i.e. NBD-FPP, a fluorescent analogue of geranylgeranyl diphosphate, which is a lipid donor for RabGGTase Rattus norvegicus ? product is mono-NBD-farnesylated Rab7:REP-1 complex ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.60 Rab geranylgeranyltransferase
-
Rattus norvegicus
2.5.1.60 RabGGTase
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.60 37
-
assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.60 0.013
-
Rab protein pH 7.2, 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.60 7.2
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.60 additional information RabGGTase can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.60 additional information
-
additional information inhibition kinetics Rattus norvegicus