BRENDA - Enzyme Database show

Design, synthesis and analysis of inhibitors of bacterial aspartate semialdehyde dehydrogenase

Cox, R.J.; Gibson, J.S.; Hadfield, A.T.; ChemBiochem 6, 2255-2260 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expression Escherichia coli
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
irreversible inhibition
Escherichia coli
1.2.1.11
(S)-2-amino-5-phosphono-pent-4-ynoic acid
-
Escherichia coli
1.2.1.11
acetylenic and z-olefinic analogues
competitive inhibition
Bacteria
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Bacteria
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Bacteria
-
-
-
1.2.1.11
Escherichia coli
P0A9Q9
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
to homogeneity
Escherichia coli
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.11
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
673074
Escherichia coli
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
673074
Bacteria
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Bacteria
1.2.1.11
NADPH
-
Bacteria
1.2.1.11
NADPH
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
1.2
-
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
Escherichia coli
1.2.1.11
3.9
-
(S)-2-amino-5-phosphono-pent-4-ynoic acid
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
Escherichia coli
1.2.1.11
20
-
z-olefin
-
Bacteria
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expression Escherichia coli
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Bacteria
1.2.1.11
NADPH
-
Bacteria
1.2.1.11
NADPH
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
irreversible inhibition
Escherichia coli
1.2.1.11
(S)-2-amino-5-phosphono-pent-4-ynoic acid
-
Escherichia coli
1.2.1.11
acetylenic and z-olefinic analogues
competitive inhibition
Bacteria
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
1.2
-
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
Escherichia coli
1.2.1.11
3.9
-
(S)-2-amino-5-phosphono-pent-4-ynoic acid
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
Escherichia coli
1.2.1.11
20
-
z-olefin
-
Bacteria
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Bacteria
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
to homogeneity
Escherichia coli
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.11
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
673074
Escherichia coli
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
673074
Bacteria
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?