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Literature summary extracted from

  • Glover, K.J.; Weerapana, E.; Numao, S.; Imperiali, B.
    Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni (2005), Chem. Biol., 12, 1311-1315.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.99.18 medicine the development of powerful inhibitors of this enzyme would be valuable in the quest for antibiotics for Campylobacter jejuni-induced gastrointestinal disorders Campylobacter jejuni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.18 preparation of a membrane fraction from Escherichia coli in which PglB has been overexpressed Campylobacter jejuni
2.4.99.19 gene pglB, expression of the wild-type and mutant WAAYGY-PglB variant in Escherichia coli in membranes Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.19 Mn2+ activates Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.18 additional information Campylobacter jejuni PglB transfers a wide variety of saccharides ?
-
 ?
2.4.99.19 tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter jejuni the native substrate for PglB is a heptasaccharide tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Campylobacter jejuni
-
-
-
2.4.99.19 Campylobacter jejuni
-
 gene pglB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 additional information PglB transfers a wide variety of saccharides Campylobacter jejuni ?
-
 ?
2.4.99.18 N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol + KDFNVSKA
-
 Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + Lys-Asp-Phe-N4-[N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-beta-D-bacillosaminyl]-Asn-Val-Ser-Lys-Ala
-
 ?
2.4.99.19 additional information ability of PglB to transfer a wide variety of saccharides and peptides, substrate specificity, mass spectrometric glycopeptide product analysis, overview. PglB readily accepts a disaccharide in vitro. PglB does require determinants in the peptide sequence beyond the canonical N-X-S/Ttripeptide Campylobacter jejuni ?
-
 ?
2.4.99.19 N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol + KDFNVSKA oligosaccharyl transferase activity of PglB in vitro with synthetic disaccharide glycan donor and peptide acceptor substrate Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + Lys-Asp-Phe-N4-[N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-beta-D-bacillosaminyl]-Asn-Val-Ser-Lys-Ala
-
 ?
2.4.99.19 tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
 Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?
2.4.99.19 tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine the native substrate for PglB is a heptasaccharide Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Synonyms

EC Number Synonyms Comment Organism
2.4.99.18 PglB
-
 Campylobacter jejuni
2.4.99.19 oligosaccharyl transferase
-
 Campylobacter jejuni
2.4.99.19 PglB
-
 Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.19 7.5
-
 assay at Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.4.99.19 malfunction if the WWDYGY signature sequence is mutated to WAAYGY, PglB is no longer active in vivo Campylobacter jejuni
2.4.99.19 metabolism PglB is involved in the general N-linked glycosylation pathway encoded by the pgl gene cluster Campylobacter jejuni
2.4.99.19 physiological function PglB catalyzes the transfer of an undecaprenyl-linked heptasaccharide to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif Campylobacter jejuni