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Literature summary extracted from

  • Kehrer, D.; Ahmed, H.; Brinkmann, H.; Siebers, B.
    Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes (2007), BMC Genet., 8, 301.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.165 additional information no increased activity in the presence of AMP, glucose, glucose 6-phosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, dihydroxyacetone phosphate, glyceraldehyde-3-phosphate, 3-phosphoglycerate, lactate, galactonate, gluconate, 2-keto-3-deoxygluconate, glucose 1-phosphate, citrate, isocitrate, alpha-ketoglutarate, succinate, fumarate, or malate Thermoproteus tenax

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.165 expressed in Escherichia coli BL21 (DE3) cells Thermoproteus tenax

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 (R)-glycerate substrate inhibition is observed at higher glycerate concentrations Thermoproteus tenax
2.7.1.165 ADP 1 mM, 91.8% activity loss, at non-saturating concentrations of ATP, competitive inhibition Thermoproteus tenax
2.7.1.165 EDTA 40 mM Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.02
-
(R)-glycerate at 70°C Thermoproteus tenax
2.7.1.165 0.03
-
ATP at 70°C Thermoproteus tenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Mg2+ 20 mM, required for highest activity Thermoproteus tenax
2.7.1.165 additional information Co2+, Mn2+, and Ni2+ (all 5 mM), and Ca2+ (2 mM) can partially replace Mg2+ (56%, 59%, 30% and 15% activity, respectively) Thermoproteus tenax
2.7.1.165 additional information K+ and NH4+ do not stimulate activity Thermoproteus tenax
2.7.1.165 additional information no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM Thermoproteus tenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 42400
-
calculated from amino acid sequence Thermoproteus tenax
2.7.1.165 44000
-
SDS-PAGE, denatured enzyme Thermoproteus tenax
2.7.1.165 48300
-
native enzyme, HiLoad 26/60 Superdex 200 prep grade column gel filtration Thermoproteus tenax

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Thermoproteus tenax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165 Q-sepharose column chromatography, phenyl sepharose column chromatography, and HiLoad 26/60 Superdex 200 prep grade gel filtration Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate
-
Thermoproteus tenax ADP + 3-phospho-(R)-glycerate
-
?
2.7.1.165 additional information ATP can be partially replaced by GTP, CTP, TTP and UTP (16%, 20%, 16%, and 10% activity, respectively) Thermoproteus tenax ?
-
?
2.7.1.165 additional information L-glycerate, galactonate, gluconate, malate, pyruvate, lactate, glyceraldehyde, glycerol, serine, 3-phosphoglycerate, ADP, diphosphate, polyphosphate, and glyceraldehyde 3-phosphate are no substrates Thermoproteus tenax ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 monomer 1 * 48300, gel filtration Thermoproteus tenax

Synonyms

EC Number Synonyms Comment Organism
2.7.1.165 More member of the multi-organism fragment with rich leucine kinase family Thermoproteus tenax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 90
-
-
Thermoproteus tenax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.165 50
-
at 50°C 15% residual activity Thermoproteus tenax
2.7.1.165 70
-
at 70°C 55% residual activity Thermoproteus tenax

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.165 ATP
-
Thermoproteus tenax