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Literature summary extracted from

  • Martinez-Martinez, I.; Navarro-Fernandez, J.; Lozada-Ramirez, J.D.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    Maximization of production of his-tagged glycine oxidase and its M261 mutant proteins (2006), Biotechnol. Prog., 22, 647-652.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 cloning of the glycine oxidase gene from Bacillus subtilis ATCC 6633 into the Rosetta Escherichia coli strain which contains the pRARE plasmid, inserting of the gene into the pET28a expression vector Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 M261H decreased in Km compared with the wild type Bacillus subtilis
1.4.3.19 M261Y decreased in Km compared with the wild type Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.18
-
 glycine M261Y mutant Bacillus subtilis
1.4.3.19 0.23
-
 sarcosine M261Y mutant Bacillus subtilis
1.4.3.19 0.29
-
 glycine-ethyl-ester M261Y mutant Bacillus subtilis
1.4.3.19 0.56
-
 glycine M261 wild type Bacillus subtilis
1.4.3.19 0.57
-
 sarcosine M261 wild type Bacillus subtilis
1.4.3.19 0.58
-
 sarcosine M261H mutant Bacillus subtilis
1.4.3.19 0.71
-
 N-ethyl-glycine M261Y mutant Bacillus subtilis
1.4.3.19 0.97
-
 glycine M261H mutant Bacillus subtilis
1.4.3.19 1.57
-
 glycine-ethyl-ester M261H mutant Bacillus subtilis
1.4.3.19 1.78
-
 glycine-ethyl-ester M261 wild type Bacillus subtilis
1.4.3.19 2.06
-
 D-proline M261Y mutant Bacillus subtilis
1.4.3.19 2.07
-
 N-ethyl-glycine M261H mutant Bacillus subtilis
1.4.3.19 2.8
-
 N-ethyl-glycine M261 wild type Bacillus subtilis
1.4.3.19 3.91
-
 D-proline M261H mutant Bacillus subtilis
1.4.3.19 8.9
-
 D-proline M261 wild type Bacillus subtilis
1.4.3.19 121.9
-
 D-alanine M261Y mutant Bacillus subtilis
1.4.3.19 155.6
-
 D-alanine M261H mutant Bacillus subtilis
1.4.3.19 315
-
 D-alanine M261 wild type Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.19 42000
-
 monomer, determined by SDS-PAGE Bacillus subtilis
1.4.3.19 172000
-
 homotetramer, determined by gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2 Bacillus subtilis
-
 pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-proline + H2O + O2 Bacillus subtilis
-
 ?
-
 ?
1.4.3.19 glycine + H2O + O2 Bacillus subtilis
-
 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine-ethyl-ester + H2O + O2 Bacillus subtilis
-
 ?
-
 ?
1.4.3.19 N-ethylglycine + H2O + O2 Bacillus subtilis
-
 glyoxylate + ethylamine + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2 Bacillus subtilis
-
 glyoxylate + methylamine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 using ultrafiltation and a HiTrap chelating affinity column Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.19 0.19
-
 crude extract Bacillus subtilis
1.4.3.19 0.21
-
 purification step ultrafiltration Bacillus subtilis
1.4.3.19 1.2
-
 purification step HiTrap chelating chromatography Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
1.4.3.19 -80°C, 75 mM sodium diphosphate buffer, pH 8.5, 10% glycerol Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2
-
 Bacillus subtilis pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-proline + H2O + O2
-
 Bacillus subtilis ?
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine-ethyl-ester + H2O + O2
-
 Bacillus subtilis ?
-
 ?
1.4.3.19 N-ethylglycine + H2O + O2
-
 Bacillus subtilis glyoxylate + ethylamine + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Bacillus subtilis glyoxylate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 homotetramer
-
 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 glycin oxidase
-
 Bacillus subtilis
1.4.3.19 GOX
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 37
-
 activity assay Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
 activity assay Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD
-
 Bacillus subtilis