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Literature summary extracted from
- Volatile fatty acid-sensing system involving coenzyme-A transferase (2006), Biotechnol. Prog., 22, 334-337.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 2.8.3.1 | biotechnology | establishing of an on-site monitoring of volatile fatty acids VFA, such as propionate, biosensing system using recombinant propionate CoA transferase and recombinant acyl-CoA oxidase, this system produces hydrogen peroxide in the presence of acetyl-CoA, oxygen, and VFA substrates, which can be quantified by colorimetric methods using peroxidase and dye reagents, e.g., 4-aminobenzoic acid plus 4-aminoantipyrine, overview | Anaerotignum propionicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.3.1 | 0.36 | - |
propionate | pH 7.0, 25°C | Anaerotignum propionicum |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.3.1 | acetyl-CoA + propionate | Anaerotignum propionicum | - |
acetate + propanoyl-CoA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.3.1 | Anaerotignum propionicum | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.3.1 | additional information | - |
development of propionate-detection systems, overview | Anaerotignum propionicum |
| 2.8.3.1 | 0.34 | - |
substrate propionate | Anaerotignum propionicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.3.1 | acetyl-CoA + butyrate | low activity | Anaerotignum propionicum | acetate + butanoyl-CoA | - |
? | |
| 2.8.3.1 | acetyl-CoA + propionate | - |
Anaerotignum propionicum | acetate + propanoyl-CoA | - |
? | |
| 2.8.3.1 | additional information | valerate is no substrate | Anaerotignum propionicum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.8.3.1 | PCT | - |
Anaerotignum propionicum |
| 2.8.3.1 | propionate CoA transferase | - |
Anaerotignum propionicum |
| 2.8.3.1 | propionate coenzyme A transferase | - |
Anaerotignum propionicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.8.3.1 | 25 | - |
assay at | Anaerotignum propionicum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.8.3.1 | 25 | 40 | over 90% of maximal activity within this range | Anaerotignum propionicum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.3.1 | 14.2 | - |
propionate | pH 7.0, 25°C | Anaerotignum propionicum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.3.1 | 7 | - |
assay at | Anaerotignum propionicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.3.1 | 6.5 | 8 | over 90% of maximal activity within this range | Anaerotignum propionicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.3.1 | acetyl-CoA | - |
Anaerotignum propionicum | |
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