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Literature summary extracted from

  • Shi, C.; Shi, X.
    Characterization of three genes encoding the subunits of light-independent protochlorophyllide reductase in Chlorella protothecoides CS-41 (2006), Biotechnol. Prog., 22, 1050-1055.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.7 expressed in Escherichia coli BL21(DE3)Lys cells Auxenochlorella protothecoides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.7.7 chloroplast
-
 Auxenochlorella protothecoides 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.7.7 38000
-
 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE Auxenochlorella protothecoides
1.3.7.7 49000
-
 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE Auxenochlorella protothecoides
1.3.7.7 58000
-
 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE Auxenochlorella protothecoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.7 chlorophyllide a + reduced ferredoxin + ATP Auxenochlorella protothecoides
-
 protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Auxenochlorella protothecoides
-
 enzyme components protein L, protein N and protein B are all transcribed constitutively independent of illumination and presence of glucose. Steady-state amounts of proteins in the light-grown cells are two- to threefold greater than in dark-grown cells. Approximately the same amount of protein is present in cultures grown mixtrophically or heterotrophically both containing glucose. Much less protein is present in photoautotrophic cells. Therefore light-independent enzyme activity depends on post-transcriptional and post-translational regulation and switches on both in light and dark
-
1.3.1.33 Auxenochlorella protothecoides CS-41
-
 enzyme components protein L, protein N and protein B are all transcribed constitutively independent of illumination and presence of glucose. Steady-state amounts of proteins in the light-grown cells are two- to threefold greater than in dark-grown cells. Approximately the same amount of protein is present in cultures grown mixtrophically or heterotrophically both containing glucose. Much less protein is present in photoautotrophic cells. Therefore light-independent enzyme activity depends on post-transcriptional and post-translational regulation and switches on both in light and dark
-
1.3.7.7 Auxenochlorella protothecoides Q6VQA8 and Q6VQA9 and Q7YKW4 subunit B, subunit L, and subunit N; strain CS-41, also named Auxenochlorella protothecoides strain CS-41
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.7 chlorophyllide a + reduced ferredoxin + ATP
-
 Auxenochlorella protothecoides protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.7.7 heterodimer 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE Auxenochlorella protothecoides

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 light-independent protochlorophyllide oxidoreductase
-
 Auxenochlorella protothecoides
1.3.1.33 LIPOR
-
 Auxenochlorella protothecoides
1.3.7.7 ChlB subunit B Auxenochlorella protothecoides
1.3.7.7 ChlL subunit L Auxenochlorella protothecoides
1.3.7.7 ChlN subunit N Auxenochlorella protothecoides
1.3.7.7 light-independent protochlorophyllide oxidoreductase
-
 Auxenochlorella protothecoides
1.3.7.7 light-independent protochlorophyllide reductase
-
 Auxenochlorella protothecoides
1.3.7.7 LIPOR
-
 Auxenochlorella protothecoides

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.7 4Fe-4S-center the enzyme contains an Fe-S cluster as redox center in all subunits Auxenochlorella protothecoides
1.3.7.7 ATP subunit L contains the ATP-binding motif Auxenochlorella protothecoides

Expression

EC Number Organism Comment Expression
1.3.7.7 Auxenochlorella protothecoides the level of theLIPOR subunit L mRNA of Chlorella protothecoides strain CS-41 is much higher in the light than in the dark up

General Information

EC Number General Information Comment Organism
1.3.7.7 physiological function light-independent protochlorophyllide reductase is required for protochlorophyllide reduction in the dark Auxenochlorella protothecoides