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Literature summary extracted from

  • Luo, T.; Peng, S.; Deng, W.; Ma, D.; Xu, Y.; Xiao, M.; Chen, F.
    Characterization of a New Stearoyl-acyl Carrier Protein Desaturase Gene from Jatropha curcas (2006), Biotechnol. Lett., 28, 657-662.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.2 cloned in pQE-30 vector and expressed in Escherichia coli strain M15, His-tag Jatropha curcas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.2 43000
-
 SDS-PAGE, overexpressed His-tag fusion protein Jatropha curcas
1.14.19.2 45510
-
 precursor Jatropha curcas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier protein] + ferredoxin + O2 Jatropha curcas
-
 oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Jatropha curcas Q4JIJ4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.2 flower
-
 Jatropha curcas
-
1.14.19.2 fruit highly expressed in developing fruits Jatropha curcas
-
1.14.19.2 husk young Jatropha curcas
-
1.14.19.2 leaf young and mature Jatropha curcas
-
1.14.19.2 root young Jatropha curcas
-
1.14.19.2 seed young Jatropha curcas
-
1.14.19.2 stem young Jatropha curcas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.2 0.000003
-
 His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, without IPTG-induction Jatropha curcas
1.14.19.2 0.000082
-
 His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, IPTG-induced Jatropha curcas
1.14.19.2 0.00157
-
 His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induction Jatropha curcas
1.14.19.2 0.00795
-
 His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induced Jatropha curcas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier protein] + ferredoxin + O2
-
 Jatropha curcas oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin reduced spinach ferredoxin Jatropha curcas