Literature summary extracted from

Noh, M.; Jung, J.H.; Lee, S.B.
Purification and characterization of glycerate kinase from the thermoacidophilic archaeon Thermoplasma acidophilum: an enzyme belonging to the second glycerate kinase family (2006), Biotechnol. Bioprocess Eng., 11, 344-350.

No PubMed abstract available

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.165	Ca2+	-	Thermoplasma acidophilum

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.165	0.32	-	ATP	pH 8.0, 55°C	Thermoplasma acidophilum
2.7.1.165	0.32	-	ATP	in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C	Thermoplasma acidophilum
2.7.1.165	0.56	-	D-glycerate	pH 8.0, 55°C	Thermoplasma acidophilum
2.7.1.165	0.56	-	(R)-glycerate	in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C	Thermoplasma acidophilum

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.1.165	Co2+	10 mM, can substitute for Mg2+, activity is 8% of the activity activated by Mg2+	Thermoplasma acidophilum
2.7.1.165	Mg2+	required	Thermoplasma acidophilum
2.7.1.165	Mg2+	10 mM, required for activity	Thermoplasma acidophilum
2.7.1.165	Mn2+	10 mM, can substitute for Mg2+, activity is 10% of the activity activated by Mg2+	Thermoplasma acidophilum
2.7.1.165	additional information	when divalent metal ions, such as Mn2+, Co2+, Ni2+, Zn2+, Ca2+, and Sr2+, are substituted for Mg2+, the enzyme activity is less than 10% of that obtained in the presence of Mg2+	Thermoplasma acidophilum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.1.165	42992	-	1 * 42992, calculated from sequence	Thermoplasma acidophilum
2.7.1.165	45000	-	SDS-PAGE, denatured enzyme	Thermoplasma acidophilum
2.7.1.165	45000	-	1 * 45000, SDS-PAGE	Thermoplasma acidophilum
2.7.1.165	45820	-	calculated from amino acid sequence	Thermoplasma acidophilum
2.7.1.165	49000	-	SDS-PAGE, native enzyme	Thermoplasma acidophilum
2.7.1.165	49300	-	gel filtration	Thermoplasma acidophilum
2.7.1.165	49300	-	Sephacryl S-200 column gel filtration	Thermoplasma acidophilum

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.165	Saccharolobus solfataricus	-	-	-
2.7.1.165	Saccharolobus solfataricus P2	-	-	-
2.7.1.165	Thermoplasma acidophilum	-	-	-
2.7.1.165	Thermoplasma acidophilum	Q9HKZ0	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.165	-	Thermoplasma acidophilum
2.7.1.165	DEAE Sepharose column chromatography, Q-Sepharose column chromatography, Phenyl-Sepharose column chromatography, and Affi-gel blue column chromatography	Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.1.165	0.16	-	crude extract, using (R)-glycerate as a substrate, at pH 8.0	Thermoplasma acidophilum
2.7.1.165	118	-	after 747fold purification, using (R)-glycerate as a substrate, at pH 8.0	Thermoplasma acidophilum
2.7.1.165	118	-	pH 8.0, 55°C	Thermoplasma acidophilum

Storage Stability

EC Number	Storage Stability	Organism
2.7.1.165	75°C, 100 mM Tris-HCl (pH 8), over 2 h, no loss of activity	Thermoplasma acidophilum
2.7.1.165	78°C, 100 mM Tris-HCl (pH 8), 1 h, 50% loss of activity	Thermoplasma acidophilum
2.7.1.165	80°C, 100 mM Tris-HCl (pH 8), 20 min, 95% loss of activity	Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.165	ATP + (R)-glycerate	-	Thermoplasma acidophilum	ADP + 3-phospho-(R)-glycerate	-	?
2.7.1.165	ATP + D-glycerate	-	Saccharolobus solfataricus	ADP + 2-phospho-D-glycerate	-	?
2.7.1.165	ATP + D-glycerate	no activity with DL-glyceraldehyde, 2-phospho-D-glycerate, 3-phospho-D-glycerate or L-tartrate. Activity with D-gluconate or glycerol at 5% of the activity compared to D-glycerate. Specificity for ATP as a phosphate donor. GTP, CTP, and UTP exhibit 0~4% of the activity of ATP	Thermoplasma acidophilum	ADP + 2-phospho-D-glycerate	-	?
2.7.1.165	ATP + D-glycerate	-	Saccharolobus solfataricus P2	ADP + 2-phospho-D-glycerate	-	?
2.7.1.165	additional information	GTP, CTP, UTP, D-glucose, D-gluconate, glycerol, D-fructose, DL-glyceraldehyde, D-ribose, D-xylose, 2-phospho-D-glycerate, 3-phospho-D-glycerate, and L-tartrate are no substrates	Thermoplasma acidophilum	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.1.165	monomer	1 * 45000, SDS-PAGE	Thermoplasma acidophilum
2.7.1.165	monomer	1 * 49000, SDS-PAGE, native enzyme	Thermoplasma acidophilum
2.7.1.165	monomer	1 * 42992, calculated from sequence	Thermoplasma acidophilum

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.1.165	Ta0453	locus name	Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.1.165	55	-	assay at	Thermoplasma acidophilum
2.7.1.165	70	-	-	Thermoplasma acidophilum
2.7.1.165	75	-	assay at	Saccharolobus solfataricus

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.7.1.165	20	85	20°C: about 40% of maximal activity, 85°C: about 50% of maximal activity	Thermoplasma acidophilum
2.7.1.165	70	80	activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C	Thermoplasma acidophilum

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.7.1.165	70	80	exhibits excellent thermal stability at 70°C, activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C, at temperatures up to 75°C, glycerate kinase is stable for over 2 h but at 78°C its activity is reduced to less than 50% in one hour, and at 80°C its activity diminishes rapidly, with less than 5% of its initial activity remaining after 20 min	Thermoplasma acidophilum
2.7.1.165	75	-	stable for over 2 h	Thermoplasma acidophilum
2.7.1.165	78	-	1 h, activity is reduced to less than 50%	Thermoplasma acidophilum
2.7.1.165	80	-	less than 5% of its initial activity remaining after 20 min	Thermoplasma acidophilum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7.1.165	8	-	assay at	Saccharolobus solfataricus
2.7.1.165	8	-	assay at	Thermoplasma acidophilum

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.7.1.165	ATP	-	Thermoplasma acidophilum

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Release 2023.1 (January 2023)