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Literature summary extracted from

  • Takarada, H.; Kawano, Y.; Hashimoto, K.; Nakayama, H.; Ueda, S.; Yohda, M.; Kamiya, N.; Dohmae, N.; Maeda, M.; Odaka, M.
    Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771 (2006), Biosci. Biotechnol. Biochem., 70, 881-889.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.84 x-ray diffraction, special focus on iron center vicinity and hydrogen bond network, similar structures of wild type and alphaQ90N mutant enzyme in nitrosylated state, resolution: 1.43 A Rhodococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.84 Q90E alpha subunit Rhodococcus sp.
4.2.1.84 Q90N alpha subunit Rhodococcus sp.
4.2.1.84 R56K beta subunit Rhodococcus sp.

General Stability

EC Number General Stability Organism
4.2.1.84 purified mutant NHases are stored in the dark without n-butyric acid, before use, NHases are activated by light irradiation. Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 n-butyric acid
-
 Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.28
-
 Methacrylonitrile mutant alphaQ90E, vmax: 280 micromol/min/mg Rhodococcus sp.
4.2.1.84 0.68
-
 Methacrylonitrile wild type, vmax: 1200 micromol/min/mg Rhodococcus sp.
4.2.1.84 1.9
-
 Methacrylonitrile mutant betaR56K, vmax: 2.5 micromol/min/mg Rhodococcus sp.
4.2.1.84 2.6
-
 Methacrylonitrile mutant alphaQ90N, vmax: 57 micromol/min/mg Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus sp.
-
-
-
4.2.1.84 Rhodococcus sp. N771
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 3 successive column chromatographies in the presence of 40 mM n-butyric acid (only wild type), alphaQ90N mutein is purified in nitrosylated state in the dark Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 methacrylonitrile + H2O
-
 Rhodococcus sp. methacrylamide
-
 ?
4.2.1.84 methacrylonitrile + H2O
-
 Rhodococcus sp. N771 methacrylamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Rhodococcus sp.
4.2.1.84 nitrile hydratase
-
 Rhodococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.84 120
-
 Methacrylonitrile mutant betaR56K Rhodococcus sp.
4.2.1.84 2600
-
 Methacrylonitrile mutant alphaQ90N Rhodococcus sp.
4.2.1.84 13000
-
 Methacrylonitrile mutant alphaQ90E Rhodococcus sp.
4.2.1.84 55000
-
 Methacrylonitrile wild type Rhodococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 2.6
-
 Butyric acid +/- 0.2, substrate: methacrylonitrile, mutant betaR56K Rhodococcus sp.
4.2.1.84 5.1
-
 Butyric acid +/- 0.3, substrate: methacrylonitrile, mutant alphaQ90E Rhodococcus sp.
4.2.1.84 6.2
-
 Butyric acid +/- 0.9, substrate: methacrylonitrile, wild type Rhodococcus sp.
4.2.1.84 14
-
 Butyric acid +/- 2.7, substrate: methacrylonitrile, mutant alphaQ90N Rhodococcus sp.