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Literature summary extracted from

  • Nakano, Y.; Okawa, S.; Yamauchi, T.; Koizumi, Y.; Sekiya, J.
    Purification and properties of soluble and bound gamma-glutamyltransferases from radish cotyledon (2006), Biosci. Biotechnol. Biochem., 70, 369-376.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
 Raphanus sativus
2.3.2.2 acivicin
-
 Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.024
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 0.032
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 0.038
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 0.038
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 0.042
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 0.042
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 0.046
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 0.046
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 0.21
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 0.22
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 0.39
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 0.41
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 0.44
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 0.47
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 0.6
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 0.66
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 2.3
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 2.4
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 4.3
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 4.4
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 cell wall bound to, enzyme form GGT A Raphanus sativus 5618
-
2.3.2.2 cell wall bound to, enzyme form GGT B Raphanus sativus 5618
-
2.3.2.2 soluble enzyme form GGT I Raphanus sativus
-
-
2.3.2.2 soluble enzyme form GGT II Raphanus sativus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 21000
-
 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
2.3.2.2 21000
-
 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
2.3.2.2 42000
-
 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
2.3.2.2 42000
-
 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
2.3.2.2 61000
-
 enzyme form GGT A, gel filtration Raphanus sativus
2.3.2.2 61000
-
 enzyme form GGT B, gel filtration Raphanus sativus
2.3.2.2 63000
-
 enzyme form GGT I, gel filtration Raphanus sativus
2.3.2.2 63000
-
 enzyme form GGT II, gel filtration Raphanus sativus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Raphanus sativus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein enzyme form GGT A Raphanus sativus
2.3.2.2 glycoprotein enzyme form GGT B Raphanus sativus
2.3.2.2 glycoprotein enzyme form GGT I Raphanus sativus
2.3.2.2 glycoprotein enzyme form GGT II Raphanus sativus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 enzyme form GGT A Raphanus sativus
2.3.2.2 enzyme form GGT B Raphanus sativus
2.3.2.2 enzyme form GGT I Raphanus sativus
2.3.2.2 enzyme form GGT II Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 cotyledon
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 0.59
-
 enzyme form GGT I Raphanus sativus
2.3.2.2 0.59
-
 enzyme form GGT II Raphanus sativus
2.3.2.2 1.48
-
 enzyme form GGT A Raphanus sativus
2.3.2.2 1.73
-
 enzyme form GGT B Raphanus sativus

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 -20°C or 4°C, activity of enzyme form GGT A is lost within 1 week Raphanus sativus
2.3.2.2 -20°C or 4°C, activity of enzyme form GGT B is lost within 1 week Raphanus sativus
2.3.2.2 -80°C, stable, enzyme form GGT I Raphanus sativus
2.3.2.2 -80°C, stable, enzyme form GGT II Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 161% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 176% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 8% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid 8% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Arg 124% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Arg 169% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Arg 44% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Arg 44% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Asp 13% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Asp 13% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Asp 140% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Asp 144% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys 7% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys-Gly 15% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys-Gly 16% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys-Gly 36% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Cys-Gly 36% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Glu 11% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Glu 13% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly 14% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly 14% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly 43% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly 48% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Met 137% of the activity with Gly-Gly, enzyme form GGT II Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Met 141% of the activity with Gly-Gly, enzyme form GGT I Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Met 43% of the activity with Gly-Gly, enzyme form GGT A Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + Met 43% of the activity with Gly-Gly, enzyme form GGT B Raphanus sativus 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
2.3.2.2 gamma-Glu-Cys + Gly-Gly
-
 Raphanus sativus Cys + 5-L-glutamyl-Gly-Gly
-
 ?
2.3.2.2 GS-bimane + Gly-Gly GS-bimane is the conjugate of GSH and monobromobimane Raphanus sativus ?
-
 ?
2.3.2.2 GSH + Gly-Gly
-
 Raphanus sativus ?
-
 ?
2.3.2.2 GSSG + Gly-Gly
-
 Raphanus sativus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE Raphanus sativus
2.3.2.2 dimer 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE Raphanus sativus
2.3.2.2 monomer 1 * 61000, enzyme form GGT A, SDS-PAGE Raphanus sativus
2.3.2.2 monomer 1 * 61000, enzyme form GGT B, SDS-PAGE Raphanus sativus

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 GGT A
-
 Raphanus sativus
2.3.2.2 GGT I
-
 Raphanus sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 11
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 12
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 13
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 13
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 15
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 16
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 20
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 21
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 23
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 27
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 30
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 30
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 31
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 31
-
 gamma-Glu-Cys 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 32
-
 GSH 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 32
-
 GSSG 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus
2.3.2.2 33
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I Raphanus sativus
2.3.2.2 38
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B Raphanus sativus
2.3.2.2 39
-
 5-L-glutamyl-4-nitroanilide 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A Raphanus sativus
2.3.2.2 40
-
 GS-bimane 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 7.5
-
 enzyme form GGT I Raphanus sativus
2.3.2.2 7.5
-
 enzyme form GGT II Raphanus sativus
2.3.2.2 7.5 8 enzyme form GGT A Raphanus sativus
2.3.2.2 7.5 8 enzyme form GGT B Raphanus sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 6 9 pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT A Raphanus sativus
2.3.2.2 6 9 pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT B Raphanus sativus
2.3.2.2 7 9 pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT I Raphanus sativus
2.3.2.2 7 9 pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT II Raphanus sativus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.2 0.016
-
 acivicin 37°C, pH 8.0, enzyme form GGT II Raphanus sativus
2.3.2.2 0.017
-
 acivicin 37°C, pH 8.0, enzyme form GGT I Raphanus sativus
2.3.2.2 0.081
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT I Raphanus sativus
2.3.2.2 0.085
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT II Raphanus sativus
2.3.2.2 0.096
-
 acivicin 37°C, pH 8.0, enzyme form GGT A Raphanus sativus
2.3.2.2 0.103
-
 acivicin 37°C, pH 8.0, enzyme form GGT B Raphanus sativus
2.3.2.2 0.47
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT B Raphanus sativus
2.3.2.2 0.57
-
 6-diazo-5-oxo-L-norleucine 37°C, pH 8.0, enzyme form GGT A Raphanus sativus