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Literature summary extracted from

  • Oba, Y.; Tanaka, K.; Inouye, S.
    Catalytic properties of domain-exchanged chimeric proteins between firefly luciferase and Drosophila fatty acyl-CoA synthetase CG6178 (2006), Biosci. Biotechnol. Biochem., 70, 2739-2744.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expressed in Escherichia coli Photinus pyralis
6.2.1.3 expressed in Escherichia coli Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 additional information a chimeric protein with the N-terminal domain of firefly luciferase and the C-terminal domain of the firefly luciferase homolog from Drosophila melanogaster CG6178 shows approximately 4% of the activity of wild type firefly luciferase Photinus pyralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 65500
-
 calculated from amino acid sequence Photinus pyralis
6.2.1.3 52500
-
 SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis P08659
-
-
6.2.1.3 Drosophila melanogaster Q9VCC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 Ni-chelate affinity chromatography Photinus pyralis
6.2.1.3 Ni-chelate affinity column chromatography Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-luciferin + O2 + ATP
-
 Photinus pyralis oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 fatty acid + ATP + CoA
-
 Photinus pyralis fatty acyl-CoA + AMP
-
 ?
1.13.12.7 luciferyl-O-adenosine monophosphate + CoA
-
 Photinus pyralis luciferyl-CoA + AMP
-
 ?
6.2.1.3 ATP + arachidonate + CoA
-
 Drosophila melanogaster AMP + diphosphate + arachidonoyl-CoA
-
 ?
6.2.1.3 ATP + decanoate + CoA
-
 Drosophila melanogaster AMP + diphosphate + decanoyl-CoA
-
 ?
6.2.1.3 ATP + laurate + CoA
-
 Drosophila melanogaster AMP + diphosphate + lauroyl-CoA
-
 ?
6.2.1.3 ATP + linoleate + CoA
-
 Drosophila melanogaster AMP + diphosphate + linoleoyl-CoA
-
 ?
6.2.1.3 ATP + octanoate + CoA
-
 Drosophila melanogaster AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA
-
 Drosophila melanogaster AMP + diphosphate + oleoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 Photinus pyralis luciferase
-
 Photinus pyralis
1.13.12.7 PpLase
-
 Photinus pyralis
6.2.1.3 CG6178
-
 Drosophila melanogaster
6.2.1.3 fatty acyl-CoA synthetase
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
 Drosophila melanogaster

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.12.7 Photinus pyralis calculated with ExPASy Compute pI/MW tool
-
 6.2
6.2.1.3 Drosophila melanogaster estimated from amino acid sequence
-
 6.5