EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.32 | CuSO4 | 0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM | Bordetella sp. | |
1.2.1.32 | HgCl2 | 0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM | Bordetella sp. | |
1.2.1.32 | iodoacetic acid | 31% inhibition at 1 mM; 69% remaining activity at an inhibitor concentration of 1 mM | Bordetella sp. | |
1.2.1.32 | N-ethylmaleimide | 18% remaining activity at an inhibitor concentration of 1 mM | Bordetella sp. | |
1.2.1.32 | N-Methylmaleimide | 82% inhibition at 1 mM | Bordetella sp. | |
1.2.1.32 | p-chloromercuribenzoic acid | 41% remaining activity at an inhibitor concentration of 1 mM; 59% inhibition at 1 mM | Bordetella sp. | |
1.2.1.32 | ZnSO4 | 26% remaining activity at an inhibitor concentration of 1 mM; 74% inhibition at 1 mM | Bordetella sp. | |
1.2.1.85 | Cu2+ | complete inhibition at 1 mM | Bordetella sp. | |
1.2.1.85 | Hg2+ | complete inhibition at 1 mM | Bordetella sp. | |
1.2.1.85 | iodoacetic acid | 69% residual activity at 1 mM | Bordetella sp. | |
1.2.1.85 | N-ethylmaleimide | 18% residual activity at 1 mM | Bordetella sp. | |
1.2.1.85 | p-chloromercuribenzoate | 41% residual activity at 1 mM | Bordetella sp. | |
1.2.1.85 | Zn2+ | 26% residual activity at 1 mM | Bordetella sp. | |
4.1.1.77 | CoCl2 | 1 mM, 47% remaining activity | Bordetella sp. | |
4.1.1.77 | FeSO4 | 1 mM, 23% remaining activity | Bordetella sp. | |
4.1.1.77 | iodoacetic acid | 1 mM, 64% remaining activity | Bordetella sp. | |
4.1.1.77 | K3Fe(CN)6 | 1 mM, 36% remaining activity | Bordetella sp. | |
4.1.1.77 | MnSO4 | 1 mM, 9% remaining activity | Bordetella sp. | |
4.1.1.77 | N-Methylmaleimide | 1 mM, 58% remaining activity | Bordetella sp. | |
4.1.1.77 | p-chloromercuribenzoic acid | 1 mM, 54% remaining activity | Bordetella sp. | |
4.1.1.77 | Tiron | 1 mM, 59% remaining activity | Bordetella sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.77 | Mg2+ | required | Bordetella sp. | |
4.2.1.80 | Mg2+ | required | Bordetella sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.77 | 29000 | - |
SDS-PAGE | Bordetella sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | Bordetella sp. | - |
2-aminomuconate + NADH + H+ | - |
? | |
1.2.1.32 | 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | Bordetella sp. | - |
2-hydroxymuconate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.32 | Bordetella sp. | - |
- |
- |
1.2.1.32 | Bordetella sp. | - |
Bordetella sp. strain 10d | - |
1.2.1.32 | Bordetella sp. 10d | - |
- |
- |
1.2.1.85 | Bordetella sp. | - |
- |
- |
1.2.1.85 | Bordetella sp. 10d | - |
- |
- |
4.1.1.77 | Bordetella sp. | - |
- |
- |
4.1.1.77 | Bordetella sp. 10d | - |
- |
- |
4.2.1.80 | Bordetella sp. | - |
- |
- |
4.2.1.80 | Bordetella sp. 10d | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.32 | ammonium sulfate fractionation, chromatography on DE52 cellulose, chromatography on DEAE-cellulofine, chromatography on DEAE-toyopearl | Bordetella sp. |
1.2.1.32 | ammonium sulfate precipitation followed by 3 chromatography steps | Bordetella sp. |
1.2.1.85 | streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography | Bordetella sp. |
4.1.1.77 | DE52 cellulose column chromatography and DEAE-Cellulofine A800 column chromatography, DEAE-Toyopearl column chromatography, streptomycin sulfate precipitation, and ammonium sulfate precipitation | Bordetella sp. |
4.2.1.80 | - |
Bordetella sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.32 | cell culture | - |
Bordetella sp. | - |
1.2.1.85 | culture condition:4-amino-3-hydroxybenzoate-grown cell | - |
Bordetella sp. | - |
1.2.1.85 | culture condition:succinate-glucose-grown cell | - |
Bordetella sp. | - |
4.1.1.77 | culture condition:4-amino-3-hydroxybenzoate-grown cell | - |
Bordetella sp. | - |
4.2.1.80 | additional information | high activity when the organism is cultivated in medium containing 0.12% (w/v) 4-amino-3-hydroxybenzoic acid as sole carbon, nitrogen, and energy source until the late exponential phase. Low activity when the organism is cultivated in succinate-glucose medium until the late-exponential phase | Bordetella sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.32 | 0.0037 | - |
after streptomycin sulfate treatment | Bordetella sp. |
1.2.1.32 | 0.0041 | - |
cell extract | Bordetella sp. |
1.2.1.32 | 0.0092 | - |
after ammonium sulfate precipitation | Bordetella sp. |
1.2.1.32 | 0.046 | - |
after DE52 chromatography | Bordetella sp. |
1.2.1.32 | 0.071 | - |
after DEAE-Toyopearl chromatography | Bordetella sp. |
1.2.1.32 | 0.075 | - |
after DEAE-Cellulofine chromatography | Bordetella sp. |
1.2.1.85 | additional information | - |
enzyme from cell extract shows a specific activity of 0.0041 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C. After 17fold purification, the enzyme shows a specific activity of 0.071 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C, in 100 mM sodium phosphate, pH 7.5, at 24°C | Bordetella sp. |
4.1.1.77 | 0.05 | - |
cell extract | Bordetella sp. |
4.1.1.77 | 18 | - |
after 360fold purification | Bordetella sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Bordetella sp. | 2-aminomuconate + NADH + H+ | - |
? | |
1.2.1.32 | 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Bordetella sp. | 2-hydroxymuconate + NADH | - |
? | |
1.2.1.32 | 2-hydroxymuconic 6-semialdehyde + NAD+ + H2O | substrate for spectrophotometric activity assay, relative activity 100% | Bordetella sp. | 2-hydroxymuconate + NADH + H+ | - |
? | |
1.2.1.32 | acetaldehyde + NAD+ + H2O | - |
Bordetella sp. | acetate + NADH + H+ | - |
? | |
1.2.1.32 | acetaldehyde + NAD+ + H2O | relative activity 113% | Bordetella sp. | acetate + NADH + H+ | - |
? | |
1.2.1.32 | acetaldehyde + NAD+ + H2O | - |
Bordetella sp. 10d | acetate + NADH + H+ | - |
? | |
1.2.1.32 | acetaldehyde + NAD+ + H2O | relative activity 113% | Bordetella sp. 10d | acetate + NADH + H+ | - |
? | |
1.2.1.32 | benzaldehyde + NAD+ + H2O | - |
Bordetella sp. | benzoate + NADH + H+ | - |
? | |
1.2.1.32 | benzaldehyde + NAD+ + H2O | relative activity 25% | Bordetella sp. | benzoate + NADH + H+ | - |
? | |
1.2.1.32 | butyraldehyde + NAD+ + H2O | relative activity 138% | Bordetella sp. | butyrate + NADH + H+ | - |
? | |
1.2.1.32 | butyraldehyde + NAD+ + H2O | relative activity 138% | Bordetella sp. 10d | butyrate + NADH + H+ | - |
? | |
1.2.1.32 | formaldehyde + NAD+ + H2O | - |
Bordetella sp. | formate + NADH | - |
? | |
1.2.1.32 | formaldehyde + NAD+ + H2O | relative activity 16,5% | Bordetella sp. | formate + NADH + H+ | - |
? | |
1.2.1.32 | n-butanal + NAD+ + H2O | - |
Bordetella sp. | n-butanoate + NADH + H+ | - |
? | |
1.2.1.32 | propanal + NAD+ + H2O | - |
Bordetella sp. | n-propanoate + NADH + H+ | - |
? | |
1.2.1.32 | propionaldehyde + NAD+ + H2O | relative activity 107% | Bordetella sp. | propionate + NADH + H+ | - |
? | |
1.2.1.32 | propionaldehyde + NAD+ + H2O | relative activity 107% | Bordetella sp. 10d | propionate + NADH + H+ | - |
? | |
1.2.1.85 | 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | 100% activity | Bordetella sp. | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+ | - |
? | |
1.2.1.85 | acetaldehyde + NAD+ + H2O | 113% activity compared to 2-hydroxymuconate-6-semialdehyde | Bordetella sp. | acetate + NADH + H+ | - |
? | |
1.2.1.85 | benzaldehyde + NAD+ + H2O | 25% activity compared to 2-hydroxymuconate-6-semialdehyde | Bordetella sp. | benzoate + NADH + H+ | - |
? | |
1.2.1.85 | butyraldehyde + NAD+ + H2O | 138% activity compared to 2-hydroxymuconate-6-semialdehyde | Bordetella sp. | butanoate + NADH + H+ | - |
? | |
1.2.1.85 | formaldehyde + NAD+ + H2O | 16.5% activity compared to 2-hydroxymuconate-6-semialdehyde | Bordetella sp. | formate + NADH + H+ | - |
? | |
1.2.1.85 | propionaldehyde + NAD+ + H2O | 107% activity compared to 2-hydroxymuconate-6-semialdehyde | Bordetella sp. | propanoate + NADH + H+ | - |
? | |
4.1.1.77 | 4-Oxalocrotonate | - |
Bordetella sp. | 2-Oxopent-4-enoate + CO2 | - |
? | |
4.1.1.77 | 4-Oxalocrotonate | - |
Bordetella sp. 10d | 2-Oxopent-4-enoate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.80 | More | 4-oxocorotonate decarboxylase and 2-oxopent-4-enoate hydratase from strain 10 days form a complex in a molar ratio 1:1 | Bordetella sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.32 | 2-hydroxymuconic 6-semialdehyde dehydrogenase | - |
Bordetella sp. |
1.2.1.32 | aminomuconate-semialdehyde dehydrogenase | - |
Bordetella sp. |
1.2.1.85 | NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase | - |
Bordetella sp. |
4.1.1.77 | 4OD | - |
Bordetella sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.32 | NAD+ | - |
Bordetella sp. |