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Literature summary extracted from
- Purification and characterization of cellobiose dehydrogenase from white-rot basidiomycete Trametes hirsuta (2006), Biosci. Biotechnol. Biochem., 70, 1629-1635.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.0013 | - |
cytochrome c | electron acceptor | Trametes hirsuta |  |
| 1.1.99.18 | 0.0122 | - |
DCPIP | electron acceptor | Trametes hirsuta | |
| 1.1.99.18 | 0.0389 | - |
3,5-di-tert-butylbenzoquinone | electron acceptor | Trametes hirsuta | |
| 1.1.99.18 | 0.042 | - |
cellobiose | electron donor | Trametes hirsuta | |
| 1.1.99.18 | 0.214 | - |
K3Fe(CN)6 | electron acceptor | Trametes hirsuta | |
| 1.1.99.18 | 0.216 | - |
cellotriose | electron donor | Trametes hirsuta | |
| 1.1.99.18 | 1.396 | - |
cellotetraose | electron donor | Trametes hirsuta | |
| 1.1.99.18 | 2.126 | - |
lactose | electron donor | Trametes hirsuta | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 91700 | - |
estimated by SDS-PAGE analysis | Trametes hirsuta |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + acceptor | Trametes hirsuta | - |
cellobiono-1,5-lactone + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Trametes hirsuta | - |
IFO 4917 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | using A POROS HP 2, gel filtation, a HI Load Superdex-200 and a Mono Q column | Trametes hirsuta |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 0.08 | - |
purification step culture filtration | Trametes hirsuta |
| 1.1.99.18 | 0.17 | - |
purification step ultra filtration | Trametes hirsuta |
| 1.1.99.18 | 1.79 | - |
purification step POROS HP2 | Trametes hirsuta |
| 1.1.99.18 | 2.64 | - |
purification step HiLoad Superdex-200 | Trametes hirsuta |
| 1.1.99.18 | 16.3 | - |
purification step Mono Q | Trametes hirsuta |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + 2,6-dichlorophenolindophenol | DCPIP, activity assay | Trametes hirsuta | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.18 | cellobiose + 3,5-di-tert-butylbenzoquinone | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone | - |
r | |
| 1.1.99.18 | cellobiose + acceptor | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellobiose + K3Fe(CN)6 | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced K3Fe(CN)6 | - |
r | |
| 1.1.99.18 | cellotetraose + acceptor | - |
Trametes hirsuta | cellotetraono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotriose + 2,6-dichlorophenolindophenol | - |
Trametes hirsuta | cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
| 1.1.99.18 | lactose + acceptor | - |
Trametes hirsuta | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | lactose + cytochrome c | - |
Trametes hirsuta | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | Cdh | - |
Trametes hirsuta |
| 1.1.99.18 | cellobiose dehydrogenase | - |
Trametes hirsuta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 40 | - |
activity assay | Trametes hirsuta |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 60 | 70 | - |
Trametes hirsuta |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 40 | - |
very stable below 40°C, above 60°C all activity is lost after 30 min | Trametes hirsuta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 5 | - |
activity assay | Trametes hirsuta |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 4 | 7 | at 40°C | Trametes hirsuta |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.99.18 | Trametes hirsuta | - |
- |
4.2 |
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