EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.81 | synthesis | biotransformation of eriodictyol using Escherichi coli expressing AtGT-2 | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.81 | expression in Escherichia coli as a glutathione S-transferase fusion protein | Arabidopsis thaliana |
2.4.1.170 | expressed in Escherichia coli | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.81 | 0.0018 | - |
eriodictyol | pH 7.0 | Arabidopsis thaliana | |
2.4.1.81 | 0.0263 | - |
kaempferol | pH 7.0 | Arabidopsis thaliana | |
2.4.1.81 | 0.0353 | - |
quercetin | pH 7.0 | Arabidopsis thaliana | |
2.4.1.170 | 0.018 | - |
eriodictyol | - |
Arabidopsis thaliana | |
2.4.1.170 | 0.0263 | - |
kaempferol | - |
Arabidopsis thaliana | |
2.4.1.170 | 0.0353 | - |
quercetin | - |
Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.81 | 54800 | - |
x * 54800, calculated from sequence | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.81 | Arabidopsis thaliana | Q8VZE9 | - |
- |
2.4.1.170 | Arabidopsis thaliana | Q8VZE9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.170 | - |
Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.81 | flower | high expression | Arabidopsis thaliana | - |
2.4.1.81 | leaf | high expression | Arabidopsis thaliana | - |
2.4.1.81 | root | - |
Arabidopsis thaliana | - |
2.4.1.81 | stem | - |
Arabidopsis thaliana | - |
2.4.1.170 | flower | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
2.4.1.170 | leaf | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
2.4.1.170 | root | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
2.4.1.170 | stem | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.170 | additional information | - |
Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.81 | UDP-alpha-D-glucose + apigenin | 29.6% of the activity with eriodictyol | Arabidopsis thaliana | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
2.4.1.81 | UDP-alpha-D-glucose + kaempferol | 78.9% of the activity with eriodictyol | Arabidopsis thaliana | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
2.4.1.81 | UDP-alpha-D-glucose + quercetin | 64.1% of the activity with eriodictyol | Arabidopsis thaliana | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
2.4.1.81 | UDP-glucose + eriodictyol | - |
Arabidopsis thaliana | UDP + 7-O-beta-D-glucosyl-eriodictyol | - |
? | |
2.4.1.81 | UDP-glucose + luteolin | 92.1% of the activity with eriodictyol | Arabidopsis thaliana | UDP + 7-O-beta-D-glucosyl-luteolin | - |
? | |
2.4.1.81 | UDP-glucose + naringenin | 71.6% of the activity with eriodictyol | Arabidopsis thaliana | UDP + 7-O-beta-D-glucosyl-naringenin | - |
? | |
2.4.1.170 | additional information | caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin. | Arabidopsis thaliana | ? | - |
? | |
2.4.1.170 | UDP-glucose + eriodictyol | - |
Arabidopsis thaliana | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + kaempferol | - |
Arabidopsis thaliana | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
2.4.1.170 | UDP-glucose + quercetin | - |
Arabidopsis thaliana | UDP + quercetin 7-O-beta-D-glucoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.81 | ? | x * 54800, calculated from sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.81 | AtGT-2 | - |
Arabidopsis thaliana |
2.4.1.170 | AtGT-2 | AtGT-2 encodes a flavonoid 7-O-glucosyltransferase | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.170 | additional information | - |
additional information | Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C | Arabidopsis thaliana |