EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.2.22 | gene astA, cloned from a genomic library, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TH2, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Eubacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.2.22 | 75000 | - |
x * 75000, cleaved recombinant enzyme, SDS-PAGE | Eubacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.22 | Eubacterium sp. | - |
- |
- |
2.8.2.22 | Eubacterium sp. A-44 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.22 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by treatement with enterokinase to exclude the DsbA and nickel affinity chromatography | Eubacterium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.2.22 | 25.6 | - |
purified, recombinant, enterokinase-cleaved enzyme | Eubacterium sp. |
2.8.2.22 | 37.1 | - |
purified recombinant enzyme | Eubacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.22 | 4-nitrophenyl sulfate + 4-hydroxybenzoate | low activity | Eubacterium sp. | 4-nitrophenol + 4-hydroxybenzoyl sulfate | - |
? | |
2.8.2.22 | 4-nitrophenyl sulfate + 4-hydroxybenzoate | low activity | Eubacterium sp. A-44 | 4-nitrophenol + 4-hydroxybenzoyl sulfate | - |
? | |
2.8.2.22 | 4-nitrophenyl sulfate + tyramine | best acceptor substrate | Eubacterium sp. | 4-nitrophenol + tyramine sulfate | - |
? | |
2.8.2.22 | 4-nitrophenyl sulfate + tyramine | best acceptor substrate | Eubacterium sp. A-44 | 4-nitrophenol + tyramine sulfate | - |
? | |
2.8.2.22 | 4-nitrophenyl sulfate + tyrosine | low activity | Eubacterium sp. | 4-nitrophenol + tyrosine sulfate | - |
? | |
2.8.2.22 | 4-nitrophenyl sulfate + tyrosine | low activity | Eubacterium sp. A-44 | 4-nitrophenol + tyrosine sulfate | - |
? | |
2.8.2.22 | additional information | the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors | Eubacterium sp. | ? | - |
? | |
2.8.2.22 | additional information | the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors | Eubacterium sp. A-44 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.2.22 | ? | x * 75000, cleaved recombinant enzyme, SDS-PAGE | Eubacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.2.22 | arylsulfate sulfotransferase | - |
Eubacterium sp. |
2.8.2.22 | ASST | - |
Eubacterium sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.2.22 | 37 | - |
assay at | Eubacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.22 | 8 | - |
assay at | Eubacterium sp. |