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Literature summary extracted from

  • Gonzalez-Segura, L.; Velasco-Garcia, R.; Rudino-Pinera, E.; Mujica-Jimenez, C.; Munoz-Clares, R.A.
    Site-directed mutagenesis and homology modeling indicate an important role of cysteine 439 in the stability of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa (2005), Biochimie, 87, 1056-1064.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.8 medicine betaine aldehyde dehydrogenase is a potential target for antimicrobial agents against Pseudomonas aeruginosa Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 C353A steady-state kinetic and structure not significantly affected Pseudomonas aeruginosa
1.2.1.8 C377A steady-state kinetic and structure not significantly affected Pseudomonas aeruginosa
1.2.1.8 C439A steady-state kinetic and structure not significantly affected, stability severely reduced Pseudomonas aeruginosa
1.2.1.8 C439S steady-state kinetic and structure not significantly affected, stability severely reduced Pseudomonas aeruginosa
1.2.1.8 C439V steady-state kinetic and structure not significantly affected, stability severely reduced Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 additional information
-
 additional information for the mutants and wild-type enzymes KM NADP+ varies within 0.060 and 0.107 mM, KM NAD+ within 0.254 and 0.411 mM, KM betaine aldehyde within 0.270 and 0.434 mM Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NAD(P)H + 2 H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 purified to homogeneity following a two-step procedure, proteins expressed in Escherichia coli Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 75
-
 mutant C439V Pseudomonas aeruginosa
1.2.1.8 102
-
 mutant C439S Pseudomonas aeruginosa
1.2.1.8 138
-
 mutant C353A Pseudomonas aeruginosa
1.2.1.8 165
-
 mutant C377A Pseudomonas aeruginosa
1.2.1.8 189
-
 mutant C439A Pseudomonas aeruginosa
1.2.1.8 196
-
 wild-type enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NAD(P)H + 2 H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.8 homotetramer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde:NAD+ oxidoreductase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.8 34.3
-
 apparent T50 mutant C439V Pseudomonas aeruginosa
1.2.1.8 37
-
 apparent T50 mutant C439S Pseudomonas aeruginosa
1.2.1.8 43.6
-
 apparent T50 mutant C439A Pseudomonas aeruginosa
1.2.1.8 46
-
 apparent T50 mutant C377A Pseudomonas aeruginosa
1.2.1.8 49.4
-
 apparent T50 mutant C353A Pseudomonas aeruginosa
1.2.1.8 50.7
-
 apparent T50 wild-type enzyme Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD(P)+
-
 Pseudomonas aeruginosa