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Literature summary extracted from

  • Krenn, B.E.; Plat, H.; Wever, R.
    Purification and some characteristics of a non-haem bromoperoxidase from Streptomyces aureofaciens (1988), Biochim. Biophys. Acta, 952, 255-260.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.18 dialysis of the enzyme preparation against 1 mM EDTA in 0.1 M citrate-phosphate buffer (pH 3.8) results in loss of enzymic activity. Incubation with vanadium, does not restore the enzymic activity. Also various other metal ions: Zn(II), Fe(II), Cu(II) and Mn(II) are ineffective in the reactivation of the preparation Kitasatospora aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.18 F-
-
 Kitasatospora aureofaciens
1.11.1.18 additional information not inhibited by azide or cyanide. Excess bromide or chloride has no effect on its brominating activity Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.18 8
-
 H2O2
-
 Kitasatospora aureofaciens
1.11.1.18 20
-
 Br-
-
 Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.18 32000
-
 2 * 32000, SDS-PAGE Kitasatospora aureofaciens
1.11.1.18 65000
-
 non-denaturing PAGE Kitasatospora aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.18 Kitasatospora aureofaciens
-
 ATCC 10762
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.18
-
 Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.18 65
-
-
 Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.18 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Kitasatospora aureofaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.18 dimer 2 * 32000, SDS-PAGE Kitasatospora aureofaciens

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 non-haem bromoperoxidase
-
 Kitasatospora aureofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.18 50
-
 activity remains constant for at least 4 h Kitasatospora aureofaciens
1.11.1.18 70
-
 slow inactivation Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.18 4.3
-
 the pH optimum is independent of H2O2 and KBr when concentrations ranging from 5 to 100 mM Kitasatospora aureofaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.18 additional information enzyme does not contain haem Kitasatospora aureofaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.18 0.00002
-
 F-
-
 Kitasatospora aureofaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.18 Kitasatospora aureofaciens isoelectric focusing, pH-range 3-10
-
 3.5