EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.5 | gene ssuD, the alkanesulfonate monooxygenase system, expressed from the ssuEADCB operon, is comprised of a flavin reductase encoded by ssuE and monooxygenase encoded by ssuD, ssuD expressionin strain BL21(DE3) | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.5 | additional information | - |
additional information | stopped-flow kinetics | Escherichia coli | |
1.14.14.5 | additional information | - |
additional information | rapid reaction kinetic analysis of SsuE-catalyzed flavin reduction using NADPH | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.38 | FMN + NADPH + H+ | Escherichia coli | - |
FMNH2 + NADP+ | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | Escherichia coli | the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation | an aldehyde + FMN + sulfite + H2O | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | Escherichia coli | the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview | an aldehyde + FMN + sulfite + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.38 | Escherichia coli | - |
- |
- |
1.14.14.5 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O | kinetic reaction mechanism, charge transfer complex formation with flavin mononucleotide reductase in the alkanesulfonate monooxygenase system | Escherichia coli | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, overview | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.38 | FMN + NADPH + H+ | - |
Escherichia coli | FMNH2 + NADP+ | - |
? | |
1.5.1.38 | FMN + NADPH + H+ | results from single-wavelength analyses at 450 and 550 nm show that reduction of FMN occurs in three distinct phases. Following a possible rapid equilibrium binding of FMN and NADPH to SsuE (MC-1) that occurs before the first detectable step, an initial fast phase (241 s-1) corresponds to the interaction of NADPH with FMN (CT-1). The second phase is a slow conversion (11 s-1) to form a charge-transfer complex of reduced FMNH2 with NADP+ (CT-2), and represents electron transfer from the pyridine nucleotide to the flavin. The third step (19 s-1) is the decay of the charge-transfer complex to SsuE with bound products (MC-2) or product release from the CT-2 complex. Results from isotope studies with [(4R)-2H]NADPH demonstrates a rate-limiting step in electron transfer from NADPH to FMN | Escherichia coli | FMNH2 + NADP+ | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | - |
Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
1.14.14.5 | an alkanesulfonate + FMNH2 + O2 | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, the FMN reductase, SsuE, catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase, SsuD, overview | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
1.14.14.5 | additional information | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, consisting of the alkanesulfonate monooxygenase and the flavin mononucleotide reductase, which catalyzes the reduction of FMN by NADPH, overview | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.38 | SsuE | - |
Escherichia coli |
1.14.14.5 | SsuD | - |
Escherichia coli |
1.14.14.5 | SsuE | - |
Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.5 | 7.5 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.5 | FMNH2 | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.1.38 | physiological function | FMN reductase (SsuE) catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase (SsuD) | Escherichia coli |