EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.2 | azide | - |
Thioalkalivibrio nitratireducens | |
1.7.2.2 | cyanide | strong inhibitor | Thioalkalivibrio nitratireducens | |
1.7.2.2 | nitrate | - |
Thioalkalivibrio nitratireducens | |
1.7.2.2 | phosphate | weak inhibitor | Thioalkalivibrio nitratireducens | |
1.7.2.2 | sulfate | weak inhibitor | Thioalkalivibrio nitratireducens | |
1.7.2.2 | sulfite | - |
Thioalkalivibrio nitratireducens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.2 | 16.4 | - |
hydroxylamine | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens | |
1.7.2.2 | 16.7 | - |
nitrite | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens | |
1.7.2.2 | 60 | - |
sulfite | in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C | Thioalkalivibrio nitratireducens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.2 | Ca2+ | 1-3 mol per subunit | Thioalkalivibrio nitratireducens | |
1.7.2.2 | Fe | 8-11 mol per subunit | Thioalkalivibrio nitratireducens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.2.2 | 64000 | - |
6 * 64000, calculated from sequence analysis and spectral properties | Thioalkalivibrio nitratireducens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.2.2 | Thioalkalivibrio nitratireducens | - |
- |
- |
1.7.2.2 | Thioalkalivibrio nitratireducens ALEN 2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.2.2 | non-denaturing preparative polyacrylamide gel electrophoresis | Thioalkalivibrio nitratireducens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.2.2 | 4080 | - |
- |
Thioalkalivibrio nitratireducens |
EC Number | Storage Stability | Organism |
---|---|---|
1.7.2.2 | 30°C, potassium phosphate buffer at pH 7.0, 24 h, no loss of activity | Thioalkalivibrio nitratireducens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.2 | hydroxylamine + ferrocytochrome c | 10 mM hydroxylamine | Thioalkalivibrio nitratireducens | NH3 + ferricytochrome c + H2O | - |
ir | |
1.7.2.2 | hydroxylamine + ferrocytochrome c | 10 mM hydroxylamine | Thioalkalivibrio nitratireducens ALEN 2 | NH3 + ferricytochrome c + H2O | - |
ir | |
1.7.2.2 | additional information | no reaction with chloride, bromate and nitrate | Thioalkalivibrio nitratireducens | ? | - |
? | |
1.7.2.2 | additional information | the enzyme possesses also peroxidase activity | Thioalkalivibrio nitratireducens | ? | - |
? | |
1.7.2.2 | additional information | no reaction with chloride, bromate and nitrate | Thioalkalivibrio nitratireducens ALEN 2 | ? | - |
? | |
1.7.2.2 | additional information | the enzyme possesses also peroxidase activity | Thioalkalivibrio nitratireducens ALEN 2 | ? | - |
? | |
1.7.2.2 | nitrite + ferrocytochrome c | - |
Thioalkalivibrio nitratireducens | NH3 + ferricytochrome c + H2O | - |
? | |
1.7.2.2 | nitrite + ferrocytochrome c | - |
Thioalkalivibrio nitratireducens ALEN 2 | NH3 + ferricytochrome c + H2O | - |
? | |
1.7.2.2 | sulfite + ? | - |
Thioalkalivibrio nitratireducens | sulfide + ? | - |
? | |
1.7.2.2 | sulfite + ? | - |
Thioalkalivibrio nitratireducens ALEN 2 | sulfide + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.2.2 | hexamer | 6 * 64000, calculated from sequence analysis and spectral properties | Thioalkalivibrio nitratireducens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.7.2.2 | ccNiR | - |
Thioalkalivibrio nitratireducens |
1.7.2.2 | cytochrome c nitrite reductase | - |
Thioalkalivibrio nitratireducens |
1.7.2.2 | multihaem c NiR | - |
Thioalkalivibrio nitratireducens |
1.7.2.2 | TvNiR | - |
Thioalkalivibrio nitratireducens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.2.2 | 80 | - |
- |
Thioalkalivibrio nitratireducens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.2.2 | 30 | - |
at an enzyme concentration of 2 mg/ml in potassium phosphate buffer pH 7.0 the enzyme is stable at 30°C for at least 24 h | Thioalkalivibrio nitratireducens |
1.7.2.2 | 50 | - |
incubation at 50°C results in 1.5fold increase of activity | Thioalkalivibrio nitratireducens |
1.7.2.2 | 70 | - |
at 70°C the enzyme looses 50% of its activity in about 3 h | Thioalkalivibrio nitratireducens |
1.7.2.2 | 80 | - |
at 80°C activity decreases by 50% in 10min | Thioalkalivibrio nitratireducens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.2.2 | 7 | 10 | - |
Thioalkalivibrio nitratireducens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.2 | heme | 7.5 units heme per subunit | Thioalkalivibrio nitratireducens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.2 | 0.01 | - |
cyanide | - |
Thioalkalivibrio nitratireducens | |
1.7.2.2 | 60 | - |
sulfite | - |
Thioalkalivibrio nitratireducens |