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Literature summary extracted from

  • Yoneda, K.; Kawakami, R.; Tagashira, Y.; Sakuraba, H.; Goda, S.; Ohshima, T.
    The first archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: Gene cloning and enzymological characterization (2006), Biochim. Biophys. Acta, 1764, 1087-1093.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.21 additional information unaffected by EDTA, CaCl2, NiCl2, CoCl2, CuSO4 or ZnCl2 Archaeoglobus fulgidus

Application

EC Number Application Comment Organism
1.4.1.21 additional information first report of an archaeal L-aspartate dehydrogenase, within the archaeal domain, homologues in many methanogenic species, but not in Thermococcales or Sulfolobales species Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.21 ligated into the expression vector pET11a, expression in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.21 0.014
-
 NADH
-
 Archaeoglobus fulgidus
1.4.1.21 0.11
-
 NAD+
-
 Archaeoglobus fulgidus
1.4.1.21 0.19
-
 L-aspartate with NAD+ as the electron acceptor Archaeoglobus fulgidus
1.4.1.21 0.32
-
 NADP+
-
 Archaeoglobus fulgidus
1.4.1.21 1.2
-
 oxaloacetate
-
 Archaeoglobus fulgidus
1.4.1.21 4.3
-
 L-aspartate with NADP+ as the electron acceptor Archaeoglobus fulgidus
1.4.1.21 167
-
 NH4+
-
 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.21 26000
-
 2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus
1.4.1.21 26208
-
 2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus
1.4.1.21 48000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.21 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.21 to homogeneity by heat treatment and affinity chromatography Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.21 4.6
-
 purified enzyme, at 50 °C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.21 L-aspartate + NAD(P)+ + H2O the enzyme shows pro-R (A-type) stereospecificity for hydrogen transfer from the C4 position of the nicotinamide moiety ofNADH Archaeoglobus fulgidus oxaloacetate + NH4+ + NAD(P)H
-
 ?
1.4.1.21 L-aspartate + NAD+
-
 Archaeoglobus fulgidus oxaloacetate + NH4+ + NADH
-
 ?
1.4.1.21 additional information no activity with D-aspartate, L-glutamate, L-alanine, L-leucine, L-phenylalanine, L-proline, glycine, L-serine, L-lysine, L-norvaline, L-norleucine, L-homoserine and L-2-amino-n-butyrate Archaeoglobus fulgidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.21 homodimer 2 * 26000, SDS-PAGE, 2 * 26208, sequence analysis Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.21 L-aspartate dehydrogenase
-
 Archaeoglobus fulgidus
1.4.1.21 L-aspDH
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.21 80
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.21 37 100
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.21 80
-
 stable for 1 h Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.21 11.6
-
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.21 NAD(P)+
-
 Archaeoglobus fulgidus