EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.6 | isozyme I subunit-encoding genes ilvB and ilvN, DNA and amino acid sequence determination and analysis, expression of His-tagged holoenzyme or individual subunits in strain BL21 | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | benzaldehyde | inhibits isozyme AHAS II, not isozyme AHAS I | Escherichia coli | |
2.2.1.6 | additional information | inhibition kinetics or recombinant wild.type and reconstituted isozymes AHAS I | Escherichia coli | |
2.2.1.6 | valine | isozyme AHAS I, cooperative feedback inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | additional information | - |
additional information | kinetics or recombinant wild-type and reconstituted isozymes AHAS I, exclusive binding model | Escherichia coli | |
2.2.1.6 | 4 | - |
pyruvate | 37°C, reconstituted, recombinant holoenzyme | Escherichia coli | |
2.2.1.6 | 4.8 | - |
pyruvate | 37°C, recombinant holoenzyme | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | Mg2+ | required | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | additional information | Escherichia coli | mechanism of expression regulation, overview | ? | - |
? | |
2.2.1.6 | pyruvate | Escherichia coli | first step in the biosynthesis of valine, overview | 2-acetolactate + CO2 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.6 | Escherichia coli | - |
isozymes I-III, subunit-encoding genes ilvB and ilvN | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.2.1.6 | recombinant His-tagged isozyme I holoenzyme or individual subunits from strain BL21 by affinity chromatography | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.2.1.6 | 2 pyruvate = 2-acetolactate + CO2 | the enzyme catalyzes the decarboxylation of bound pyruvate to form a hydroxyethyl-thiamine diphosphate anion/enamine intermediate, which normally reacts with a second molecule of an alpha-ketoacid to form an acetohydroxyacid, but can also perform several side reactions proceeding through the hydroxyethyl-thiamine diphosphate anion/enamine intermediate | Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.2.1.6 | reconstitution of the holoenzyme from recombinantly expressed, purified subunits encoded by genes ilvB and ilvN | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.2.1.6 | additional information | - |
- |
Escherichia coli |
2.2.1.6 | 60.5 | - |
reconstituted, recombinant holoenzyme | Escherichia coli |
2.2.1.6 | 63.6 | - |
recombinant holoenzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | additional information | mechanism of expression regulation, overview | Escherichia coli | ? | - |
? | |
2.2.1.6 | additional information | acetohydroxybutyrate is preferably formed, isozyme AHAS I can also form peracetate from synthetic acetolactate | Escherichia coli | ? | - |
? | |
2.2.1.6 | pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
r | |
2.2.1.6 | pyruvate | first step in the biosynthesis of valine, overview | Escherichia coli | 2-acetolactate + CO2 | - |
r | |
2.2.1.6 | pyruvate + benzaldehyde | stereospecific reaction, isozymes AHAS I and II | Escherichia coli | (R)-phenylacetylcarbinol + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.6 | acetohydroxyacid synthase | - |
Escherichia coli |
2.2.1.6 | AHAS | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.6 | 37 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | FAD | - |
Escherichia coli | |
2.2.1.6 | thiamine diphosphate | dependent on | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 1.4 | - |
benzaldehyde | isozyme AHAS II | Escherichia coli |