EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.7.7 | expressed in Rhodobacter capsulatus mutant DB176 cells | Rhodobacter capsulatus |
EC Number | General Stability | Organism |
---|---|---|
1.3.1.33 | rapid loss of activity during attempts to purify | Rhodobacter capsulatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.33 | additional information | - |
additional information | stable enzyme assay system by mixing recombinant enzyme components L-protein and NB-protein under anaerobic conditiions | Rhodobacter capsulatus | |
1.3.1.33 | 0.0106 | - |
protochlorophyllide | pH 7.4, 34°C | Rhodobacter capsulatus | |
1.3.7.7 | 0.0106 | - |
protochlorophyllide | in 100 mM HEPES-KOH (pH 7.4), 5 mM MgCl2, 5 mM dithiothreitol, at 34°C | Rhodobacter capsulatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.33 | 36000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | 36046 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | 48671 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | 52000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | 57191 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | 60000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.7.7 | 36000 | - |
2 * 36000, S-tag subunit BchL, SDS-PAGE | Rhodobacter capsulatus |
1.3.7.7 | 36046 | - |
2 * 36046, subunit BchL calculated from amino acid sequence | Rhodobacter capsulatus |
1.3.7.7 | 48671 | - |
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence | Rhodobacter capsulatus |
1.3.7.7 | 52000 | - |
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE | Rhodobacter capsulatus |
1.3.7.7 | 57191 | - |
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence | Rhodobacter capsulatus |
1.3.7.7 | 60000 | - |
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE | Rhodobacter capsulatus |
1.3.7.7 | 67000 | - |
S-tag subunit BchL, gel filtration | Rhodobacter capsulatus |
1.3.7.7 | 200000 | - |
NB-protein complex, gel filtration | Rhodobacter capsulatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.33 | Rhodobacter capsulatus | - |
- |
- |
1.3.7.7 | Rhodobacter capsulatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.33 | both activities of the components protein L and proteins NB are rapidly lost during purification procedures, such as affinity chromatography with S-protein agarose. Attempts to purify the active components have so far been unsuccessful | Rhodobacter capsulatus |
1.3.7.7 | S-protein agarose column chromatography | Rhodobacter capsulatus |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.33 | enzyme in crude extracts stable for more than 6 months when maintained anaerobically at 4°C, with no significant loss of activity | Rhodobacter capsulatus |
1.3.7.7 | 4°C, NB- and L-protein subunits of the crude extract when maintained anaerobically, more than 6 months, remain stable with no significant loss of activity | Rhodobacter capsulatus |
1.3.7.7 | 4°C, NB- and L-protein subunits of the purified extract when maintained anaerobically, both activities of the components are rapidly lost during purification procedures such as affinity chromatography with S-protein agarose | Rhodobacter capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.33 | protochlorophyllide + NADPH | - |
Rhodobacter capsulatus | chlorophyllide + NADP+ | - |
? | |
1.3.7.7 | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
Rhodobacter capsulatus | chlorophyllide a + reduced ferredoxin + ATP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.33 | ? | x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
1.3.1.33 | More | recombinant enzyme component L-protein forms a dimer, recombinant components NB-protein form a heterotetramer, gel filtration | Rhodobacter capsulatus |
1.3.7.7 | heterotetramer | 2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence | Rhodobacter capsulatus |
1.3.7.7 | heterotetramer | 2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE | Rhodobacter capsulatus |
1.3.7.7 | homodimer | 2 * 36000, S-tag subunit BchL, SDS-PAGE | Rhodobacter capsulatus |
1.3.7.7 | homodimer | 2 * 36046, subunit BchL calculated from amino acid sequence | Rhodobacter capsulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.33 | DPOR | - |
Rhodobacter capsulatus |
1.3.7.7 | dark-operative protochlorophyllide reductase | - |
Rhodobacter capsulatus |
1.3.7.7 | DPOR | - |
Rhodobacter capsulatus |
1.3.7.7 | light-independent Pchlide oxidoreductase | - |
Rhodobacter capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.33 | Ferredoxin | - |
Rhodobacter capsulatus | |
1.3.7.7 | ADP | - |
Rhodobacter capsulatus |