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Literature summary extracted from

  • Vasilev, V.I.; Tikhonova, T.V.; Gvozdev, R.I.; Tukhvatullin, I.A.; Popov, V.O.
    Optimization of solubilization and purification procedures for the hydroxylase component of membrane-bound methane monooxygenase from Methylococcus capsulatus strain M (2006), Biochemistry (Moscow), 71, 1329-1335.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 duroquinol increasing duroquinol concentration above 70 mM causes almost total inhibition of enzyme activity Methylococcus capsulatus
1.14.18.3 NaCl decrease in activity might be due to reduced enzyme solubility with increasing NaCl concentrations Methylococcus capsulatus
1.14.18.3 propylene oxide product inhibition at higher concentration Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane bound Methylococcus capsulatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 Co2+ the enzyme contains three Co2+ ions per enzyme molecule Methylococcus capsulatus
1.14.18.3 Fe2+ the enzyme contains one Fe2+ ion per enzyme molecule Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 23000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 26000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 47000
-
 x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 660000
-
 solubilized enzyme, gel filtration Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2 Methylococcus capsulatus
-
 methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2 Methylococcus capsulatus M
-
 methanol + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus M
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 optimization of solubilization and purification procedure for the hydroxylase component of membrane-bound enzyme, purification to homogeneity involves solubilization by dodexylbeta-D-maltoside, ion exchange chromatography and gel filtration, the purification includes the loss of the reductase component Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.3 additional information
-
 optimization of a coupled assay method with propylene as substrate and a duroquinol-based NADH regeneration system Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus M methanol + acceptor + H2O
-
 ?
1.14.18.3 Mn2+ + H2O2
-
 Methylococcus capsulatus ?
-
 ?
1.14.18.3 Mn2+ + H2O2
-
 Methylococcus capsulatus M ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 oligomer x * 47000 + x * 26000 + x * 23000, SDS-PAGE Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 MMO
-
 Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.3 40 45
-
 Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.3 7.2 7.3 assay at Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
 Methylococcus capsulatus