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Literature summary extracted from

  • Bonina, T.A.; Gilep, A.A.; Estabrook, R.W.; Usanov, S.A.
    Engineering of proteolytically stable NADPH-cytochrome P450 reductase (2005), Biochemistry, 70, 357-365.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 wild-type and mutant K56Q, expression in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.4 K56Q the full-length mutant enzyme is stable to spontaneopus proteolysis but possesses spectral and catzalytic properties of the wild-type flavoprotein Rattus norvegicus
1.6.2.4 additional information The removal of the N-terminal hydrophobic sequence of NADPH-cytochrome P450 reductase results in loss of the ability of the flavoprotein to interact and transfer electrons to cytochrome P450. Truncated forms of the flavoprotein (residue 46-676 of the mutant (Q56Q) or 57-676 of the wild-type NADPH-cytochrome P450 reductase) are unable to transfer electrons to cytochrome P450c17 or P4503A4 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 membrane bound to Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH Rattus norvegicus NADPH-cytochrome P450 reductase is a unique universal donor of electrons to practically all known microsomal cytochrome P450s and one of the most important components of the monooxygenase system 2 ferrocytochrome c + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 recombinant Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH NADPH-cytochrome P450 reductase is a unique universal donor of electrons to practically all known microsomal cytochrome P450s and one of the most important components of the monooxygenase system Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 NADPH
-
 Rattus norvegicus