BRENDA - Enzyme Database

Mechanistic characterization of the bifunctional aminoglycoside-modifying enzyme AAC(3)-Ib/AAC(6)-Ib from Pseudomonas aeruginosa

Kim, C.; Villegas-Estrada, A.; Hesek, D.; Mobashery, S.; Biochemistry 46, 5270-5282 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.82
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
butyryl-CoA
-
Pseudomonas aeruginosa
2.3.1.82
CoASH
-
Pseudomonas aeruginosa
2.3.1.82
N6'-acetyl kanamycin A
-
Pseudomonas aeruginosa
2.3.1.82
paromomycin
-
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.0003
-
isepamicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
kanamycin A
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
amikacin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
netilmicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.0012
-
neomycin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.003
-
dibekacin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.0083
-
gentamicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.009
-
acetyl-CoA
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.82
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + amikacin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + isepamicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylisepamicin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylneomycin
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
additional information
no activity with fortimicin A. Bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b'?domain exhibits a broad substrate spectrum
672253
Pseudomonas aeruginosa
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.82
AAC(3)-Ib/AAC(6')-Ib'
bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b' domain (EC 2.3.1.82) exhibits a broad substrate spectrum
Pseudomonas aeruginosa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.82
1
-
acetyl-CoA
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
1.3
-
netilmicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
1.3
-
dibekacin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2
-
gentamicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2.4
-
kanamycin A
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2.4
-
isepamicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
3.5
-
amikacin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
6
-
neomycin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.0012
-
paromomycin
versus kanamycin A, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.02
-
CoASH
versus kanamycin A. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.03
-
CoASH
versus acetyl-CoA, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.04
-
paromomycin
versus acetyl-CoA, K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.04
-
6'-N-acetylated kanamycin A
versus acetyl-CoA. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.05
-
6'-N-acetylated kanamycin A
versus kanamycin A. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.11
-
butyryl-CoA
versus acetyl-CoA, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.15
-
butyryl-CoA
versus kanamycin A, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.2
-
CoASH
versus kanamycin A. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.38
-
6'-N-acetylated kanamycin A
versus acetyl-CoA. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.55
-
6'-N-acetylated kanamycin A
versus kanamycin A. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.57
-
butyryl-CoA
versus kanamycin A, K(ii)
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
butyryl-CoA
-
Pseudomonas aeruginosa
2.3.1.82
CoASH
-
Pseudomonas aeruginosa
2.3.1.82
N6'-acetyl kanamycin A
-
Pseudomonas aeruginosa
2.3.1.82
paromomycin
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.0012
-
paromomycin
versus kanamycin A, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.02
-
CoASH
versus kanamycin A. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.03
-
CoASH
versus acetyl-CoA, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.04
-
paromomycin
versus acetyl-CoA, K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.04
-
6'-N-acetylated kanamycin A
versus acetyl-CoA. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.05
-
6'-N-acetylated kanamycin A
versus kanamycin A. K(is)
Pseudomonas aeruginosa
2.3.1.82
0.11
-
butyryl-CoA
versus acetyl-CoA, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.15
-
butyryl-CoA
versus kanamycin A, K(is)
Pseudomonas aeruginosa
2.3.1.82
0.2
-
CoASH
versus kanamycin A. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.38
-
6'-N-acetylated kanamycin A
versus acetyl-CoA. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.55
-
6'-N-acetylated kanamycin A
versus kanamycin A. K(ii)
Pseudomonas aeruginosa
2.3.1.82
0.57
-
butyryl-CoA
versus kanamycin A, K(ii)
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.0003
-
isepamicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
kanamycin A
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
amikacin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.001
-
netilmicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.0012
-
neomycin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.003
-
dibekacin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.0083
-
gentamicin
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
0.009
-
acetyl-CoA
reaction with AAC (6?)-Ib' domain
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + amikacin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + isepamicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylisepamicin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylneomycin
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
672253
Pseudomonas aeruginosa
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
additional information
no activity with fortimicin A. Bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b'?domain exhibits a broad substrate spectrum
672253
Pseudomonas aeruginosa
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.82
1
-
acetyl-CoA
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
1.3
-
netilmicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
1.3
-
dibekacin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2
-
gentamicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2.4
-
kanamycin A
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
2.4
-
isepamicin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
3.5
-
amikacin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa
2.3.1.82
6
-
neomycin
reaction with AAC (6')-Ib' domain
Pseudomonas aeruginosa