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Literature summary extracted from

  • Kim, C.; Villegas-Estrada, A.; Hesek, D.; Mobashery, S.
    Mechanistic characterization of the bifunctional aminoglycoside-modifying enzyme AAC(3)-Ib/AAC(6)-Ib from Pseudomonas aeruginosa (2007), Biochemistry, 46, 5270-5282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.82 butyryl-CoA
-
 Pseudomonas aeruginosa
2.3.1.82 CoASH
-
 Pseudomonas aeruginosa
2.3.1.82 N6'-acetyl kanamycin A
-
 Pseudomonas aeruginosa
2.3.1.82 paromomycin
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.0003
-
 isepamicin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.001
-
 kanamycin A reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.001
-
 amikacin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.001
-
 netilmicin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.0012
-
 neomycin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.003
-
 dibekacin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.0083
-
 gentamicin reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa
2.3.1.82 0.009
-
 acetyl-CoA reaction with AAC (6?)-Ib' domain Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
 Pseudomonas aeruginosa CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Pseudomonas aeruginosa CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin
-
 Pseudomonas aeruginosa CoA + N6'-acetylgentamicin
-
 ?
2.3.1.82 acetyl-CoA + isepamicin
-
 Pseudomonas aeruginosa CoA + N6'-acetylisepamicin
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Pseudomonas aeruginosa CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + neomycin
-
 Pseudomonas aeruginosa CoA + N6'-acetylneomycin
-
 ?
2.3.1.82 acetyl-CoA + netilmicin
-
 Pseudomonas aeruginosa CoA + N6'-acetylnetilmicin
-
 ?
2.3.1.82 additional information no activity with fortimicin A. Bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b'?domain exhibits a broad substrate spectrum Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(3)-Ib/AAC(6')-Ib' bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b' domain (EC 2.3.1.82) exhibits a broad substrate spectrum Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 1
-
 acetyl-CoA reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 1.3
-
 netilmicin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 1.3
-
 dibekacin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 2
-
 gentamicin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 2.4
-
 kanamycin A reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 2.4
-
 isepamicin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 3.5
-
 amikacin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa
2.3.1.82 6
-
 neomycin reaction with AAC (6')-Ib' domain Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.82 0.0012
-
 paromomycin versus kanamycin A, K(is) Pseudomonas aeruginosa
2.3.1.82 0.02
-
 CoASH versus kanamycin A. K(is) Pseudomonas aeruginosa
2.3.1.82 0.03
-
 CoASH versus acetyl-CoA, K(is) Pseudomonas aeruginosa
2.3.1.82 0.04
-
 paromomycin versus acetyl-CoA, K(ii) Pseudomonas aeruginosa
2.3.1.82 0.04
-
 6'-N-acetylated kanamycin A versus acetyl-CoA. K(is) Pseudomonas aeruginosa
2.3.1.82 0.05
-
 6'-N-acetylated kanamycin A versus kanamycin A. K(is) Pseudomonas aeruginosa
2.3.1.82 0.11
-
 butyryl-CoA versus acetyl-CoA, K(is) Pseudomonas aeruginosa
2.3.1.82 0.15
-
 butyryl-CoA versus kanamycin A, K(is) Pseudomonas aeruginosa
2.3.1.82 0.2
-
 CoASH versus kanamycin A. K(ii) Pseudomonas aeruginosa
2.3.1.82 0.38
-
 6'-N-acetylated kanamycin A versus acetyl-CoA. K(ii) Pseudomonas aeruginosa
2.3.1.82 0.55
-
 6'-N-acetylated kanamycin A versus kanamycin A. K(ii) Pseudomonas aeruginosa
2.3.1.82 0.57
-
 butyryl-CoA versus kanamycin A, K(ii) Pseudomonas aeruginosa