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Literature summary extracted from

  • Zhang, L.; Cooper, A.J.; Krasnikov, B.F.; Xu, H.; Bubber, P.; Pinto, J.T.; Gibson, G.E.; Hanigan, M.H.
    Cisplatin-induced toxicity is associated with platinum deposition in mouse kidney mitochondria in vivo and with selective inactivation of the alpha-ketoglutarate dehydrogenase complex in LLC-PK1 cells (2006), Biochemistry, 45, 8959-8971.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.105 medicine susceptibility of KGDHC to inactivation in kidney cells exposed to cisplatin metabolites my be due to the proximity of mitochondrial aspartate aminotransferase to KGDHC in mitochondria, mitochondrial aspartate aminotransferase may catalyze a beta-lyase reaction with the cisplatin-cysteine S-conjugate, converting cisplatin to a toxicant Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.105 cisplatin treatment with 0.05 or 0.1 mM for 3 h, followed by removal of cisplatin from the medium for 24 h, results in a pronounced loss of activity, both in mitochondrial aspartate aminotransferase-transfected cells and control cells, exposure to 0.1 mM results in a significantly greater loss of activity in mitochondrial aspartate aminotransferase-transfected cells than in control cells Sus scrofa
4.4.1.13 aminooxyacetic acid
-
 Mus musculus
4.4.1.13 aminooxyacetic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.105 mitochondrion
-
 Sus scrofa 5739
-
4.4.1.13 cytosol
-
 Mus musculus 5829
-
4.4.1.13 microsome
-
 Mus musculus
-
-
4.4.1.13 mitochondrion
-
 Mus musculus 5739
-
4.4.1.13 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.105 Sus scrofa
-
-
-
4.4.1.13 Mus musculus
-
-
-
4.4.1.13 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.105 kidney
-
 Sus scrofa
-
1.2.1.105 LLC-PK1 cell
-
 Sus scrofa
-
4.4.1.13 kidney
-
 Mus musculus
-
4.4.1.13 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 25
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 (H2N)2Pt(Cl)SCH2CH(NH3+)CO2- + H2O
-
 Mus musculus CH3C(O)CO2- + NH4+ + (NH2)2Pt(Cl)SH
-
 ?
4.4.1.13 (H2N)2Pt(Cl)SCH2CH(NH3+)CO2- + H2O
-
 Rattus norvegicus CH3C(O)CO2- + NH4+ + (NH2)2Pt(Cl)SH
-
 ?
4.4.1.13 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
 Mus musculus ?
-
 ?
4.4.1.13 S-(1,2-dichlorovinyl)-L-cysteine
-
 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.105 alpha-ketoglutarate dehydrogenase complex
-
 Sus scrofa
1.2.1.105 KGDHC
-
 Sus scrofa
4.4.1.13 cysteine S-conjugate beta-lyase
-
 Mus musculus
4.4.1.13 cysteine S-conjugate beta-lyase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate
-
 Mus musculus
4.4.1.13 pyridoxal 5'-phosphate
-
 Rattus norvegicus