EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.1 | into the pVL1393 vector | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.99.1 | Y348F | mutant | Homo sapiens |
1.14.99.1 | Y348F/Y504F | mutant | Homo sapiens |
1.14.99.1 | Y504F | mutant | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.1 | aspirin | - |
Homo sapiens | |
1.14.99.1 | nimesulide | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.99.1 | 0.0021 | - |
arachidonic acid | wild-type, cyclooxygenase activity | Homo sapiens | |
1.14.99.1 | 0.0034 | - |
arachidonic acid | mutant Y348F/Y504F, cyclooxygenase activity | Homo sapiens | |
1.14.99.1 | 0.0038 | - |
arachidonic acid | mutant Y348F, cyclooxygenase activity | Homo sapiens | |
1.14.99.1 | 0.0042 | - |
arachidonic acid | mutant Y504F, cyclooxygenase activity | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.99.1 | membrane | - |
Homo sapiens | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.99.1 | 73000 | - |
determined by SDS-PAGE and immunoblotting | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.1 | arachidonate + AH2 + O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.1 | - |
Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.99.1 | 0.0027 | - |
mutant Y348F, cyclooxygenase activity | Homo sapiens |
1.14.99.1 | 0.0039 | - |
mutant Y504F, cyclooxygenase activity | Homo sapiens |
1.14.99.1 | 0.0047 | - |
mutant Y348F/Y504F, cyclooxygenase activity | Homo sapiens |
1.14.99.1 | 0.0058 | - |
wild-type, cyclooxygenase activity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.1 | arachidonate + AH2 + O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.1 | hPGHS-2 | - |
Homo sapiens |
1.14.99.1 | PGHS | - |
Homo sapiens |
1.14.99.1 | PGHS-2 | - |
Homo sapiens |
1.14.99.1 | prostaglandin H synthase | - |
Homo sapiens |
1.14.99.1 | prostaglandin H synthase-2 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.1 | 22 | - |
lipoxygenase activity assay at room temperature | Homo sapiens |
1.14.99.1 | 30 | - |
cyclooxygenase activity assay | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.99.1 | 8 | - |
lipoxygenase activity assay | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.1 | heme | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.99.1 | 0.00027 | - |
nimesulide | mutant Y348F/Y504F, cyclooxygenase inhibition | Homo sapiens | |
1.14.99.1 | 0.0009 | - |
nimesulide | mutant Y504F, cyclooxygenase inhibition | Homo sapiens | |
1.14.99.1 | 0.001 | - |
nimesulide | wild-type, cyclooxygenase inhibition | Homo sapiens |