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Literature summary extracted from

  • Pandini, V.; Caprini, G.; Tedeschi, G.; Seeber, F.; Zanetti, G.; Aliverti, A.
    Roles of the species-specific subdomain and the N-terminal peptide of Toxoplasma gondii ferredoxin-NADP+ reductase in ferredoxin binding (2006), Biochemistry, 45, 3563-3571.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 DELTA1-15 removal of the N-terminal 15 residues of enzyme enhances the interaction with ferredoxin Toxoplasma gondii
1.18.1.2 DELTA81-118 deleted amino acid comprise a species-specific subdomain containing an insertion of 28 residues, deletion results in an catalytically active enzyme form with 10fold decreased affinity for ferredoxin Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0028
-
 reduced ferredoxin mutant lacking amino acids 81 to 118, pH 8.2, 25°C Toxoplasma gondii
1.18.1.2 0.015
-
 NADPH wild-type, pH 9.0, 25°C Toxoplasma gondii
1.18.1.2 0.016
-
 NADPH mutant lacking amino acids 81 to 118, pH 9.0, 25°C Toxoplasma gondii
1.18.1.2 0.018
-
 reduced ferredoxin wild-type, pH 8.2, 25°C Toxoplasma gondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 35000
-
 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
1.18.1.2 37600
-
 gel filtration, mutant lacking amino acids 81 to 118 Toxoplasma gondii
1.18.1.2 39000
-
 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
1.18.1.2 46300
-
 gel filtration, wildtype Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Toxoplasma gondii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 oxidized ferredoxin + NADPH
-
 Toxoplasma gondii reduced ferredoxin + NADP+
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Toxoplasma gondii oxidized ferredoxin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
1.18.1.2 More study on enzyme conformation by limited proteolysis. Protein substrate ferredoxin protects ferredoxin-NADP+ reductase against cleavage both at its N-terminal peptide and at its largest sequence insertion of 28 residues Toxoplasma gondii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 130
-
 reduced ferredoxin mutant lacking pH 8.2, 25°C Toxoplasma gondii
1.18.1.2 260
-
 reduced ferredoxin wild-type, pH 8.2, 25°C Toxoplasma gondii
1.18.1.2 424
-
 NADPH mutant lacking pH 9.0, 25°C Toxoplasma gondii
1.18.1.2 540
-
 NADPH wild-type, pH 9.0, 25°C Toxoplasma gondii