Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fagan, R.L.; Nelson, M.N.; Pagano, P.M.; Palfey, B.A.
    Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases (2006), Biochemistry, 45, 14926-14932.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.5.2 additional information kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, are about 3fold for DHO labeled at the 5-position, about 4fold for DHO labeled at the 6-position, and about 6-7fold for DHO labeled at both the 5- and 6-positions, at a pH value above the pKa controlling reduction, no isotope effect was observed for DHO deuterated at the 5-position, which is consistent with a stepwise reaction, above the kinetic pKa, the deprotonation of C5 is fast enough that it does not contribute to the observed rate constant and, therefore, is not isotopically sensitive Escherichia coli
1.3.5.2 additional information kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, pKa near 9.4 controlling reduction, similar to that previously reported for the Escherichia coli enzyme Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2
-
 Escherichia coli
1.3.5.2
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Escherichia coli
-
-
-
1.3.5.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2
-
 Escherichia coli
1.3.5.2
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 dihydroorotate + acceptor
-
 Escherichia coli orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor
-
 Homo sapiens orotate + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.5.2 class 2 dihydroorotate dehydrogenases
-
 Escherichia coli
1.3.5.2 class 2 dihydroorotate dehydrogenases
-
 Homo sapiens
1.3.5.2 DHODH
-
 Escherichia coli
1.3.5.2 DHODH
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.5.2 6 11.3 the rate constant for flavin reduction increases with pH Homo sapiens
1.3.5.2 6.5 8.5
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 flavin
-
 Escherichia coli
1.3.5.2 flavin
-
 Homo sapiens