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Literature summary extracted from

  • Manna, S.K.; Mazumdar, S.
    Role of threonine 101 on the stability of the heme active site of cytochrome P450cam: multiwavelength circular dichroism studies (2006), Biochemistry, 45, 12715-12722.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 gene camC, expression of mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 T101V site-directed mutagenesis, the mutant shows decreased thermal stability of the heme active site and reaction intermediates in the reaction, equilibrium unfolding compared to the wild-type enzyme Pseudomonas putida

General Stability

EC Number General Stability Organism
1.14.15.1 Thr101 is required for the enzyme reaction intermediate Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.1 additional information
-
 additional information thermal unfolding kinetics, substrate binding kinetics Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ heme iron, located in the active site, Thr101 is important for stability, tertiary and secondary structure of the heme active site, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183 strain containing the C334A mutant enzyme, gene camC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.1 More tertiary and secondary structure of the heme active site, overview Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.1 additional information
-
 thermal unfolding data, thermal multistep unfolding modell, Thr101 is important for thermal stability, equilibrium unfolding of wild-type C334A and mutant enzymes Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin
-
 Pseudomonas putida