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Literature summary extracted from

  • Marohnic, C.C.; Crowley, L.J.; Davis, C.A.; Smith, E.T.; Barber, M.J.
    Cytochrome b5 reductase: role of the si-face residues, proline 92 and tyrosine 93, in structure and catalysis (2005), Biochemistry, 44, 2449-2461.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 P92A mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 P92G mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 P92S mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93A mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93D mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93F mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93H mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93S mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus
1.6.2.2 Y93W mutation preceeding the conserved motif RxYTSxxSN, FAD is bound in 1:1 cofactor:protein stoichiometry Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0038
-
 NADH mutant Y93S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0048
-
 NADH mutant P92A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0049
-
 NADH mutant Y93D, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0058
-
 ferricyanide mutant Y93S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.006
-
 NADH wild-type, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0065
-
 NADH mutant P92G, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0066
-
 ferricyanide mutant Y93F, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0067
-
 NADH mutant Y93F, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0068
-
 ferricyanide mutant Y93W, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0071
-
 ferricyanide wild-type, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0073
-
 NADH mutant Y93A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0074
-
 ferricyanide mutant P92A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0077
-
 NADH mutant P92S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0078
-
 ferricyanide mutant P92S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.008
-
 ferricyanide mutant P92G, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0083
-
 ferricyanide mutant Y93D, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0083
-
 ferricyanide mutant Y93H, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0086
-
 ferricyanide mutant Y93A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.0088
-
 NADH mutant Y93H, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.01
-
 ferricytochrome b5 mutant Y93D, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.01
-
 ferricytochrome b5 mutant Y93H, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.011
-
 ferricytochrome b5 mutant Y93F, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.011
-
 ferricytochrome b5 mutant Y93W, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.012
-
 ferricytochrome b5 mutant P92G, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.012
-
 ferricytochrome b5 mutant P92S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.012
-
 ferricytochrome b5 mutant Y93S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.013
-
 ferricytochrome b5 wild-type, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.014
-
 ferricytochrome b5 mutant Y93A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.015
-
 ferricytochrome b5 mutant P92A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 0.054
-
 NADH mutant Y93W, pH 7.0, 25°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Rattus norvegicus P20070
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.2.2 NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 Y93 forms contact with the FAD, but neither P92 nor Y93 preceeding the conserved motif RxYTSxxSN coordinating flavin binding are critical for flavin incorporation Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Rattus norvegicus 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 14
-
 ferricytochrome b5 mutant Y93D, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 19
-
 ferricytochrome b5 mutant Y93W, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 27
-
 ferricytochrome b5 mutant Y93H, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 32
-
 ferricytochrome b5 mutant Y93S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 57
-
 ferricytochrome b5 mutant Y93A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 83
-
 ferricyanide mutant Y93D, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 83
-
 ferricyanide mutant Y93H, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 83
-
 ferricyanide mutant Y93W, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 133
-
 ferricyanide mutant Y93S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 200
-
 ferricyanide mutant Y93A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 295
-
 ferricytochrome b5 mutant Y93F, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 315
-
 ferricytochrome b5 mutant P92S, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 320
-
 ferricytochrome b5 mutant P92A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 335
-
 ferricytochrome b5 mutant P92G, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 400
-
 ferricytochrome b5 wild-type, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 467
-
 ferricyanide mutant P92G, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 483
-
 ferricyanide mutant Y93F, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 517
-
 ferricyanide mutant P92A, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 800
-
 ferricyanide wild-type, pH 7.0, 25°C Rattus norvegicus
1.6.2.2 880
-
 ferricyanide mutant P92S, pH 7.0, 25°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Rattus norvegicus