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Literature summary extracted from

  • Fang, Q.; Kanugula, S.; Pegg, A.E.
    Function of domains of human O6-alkylguanine-DNA alkyltransferase (2005), Biochemistry, 44, 15396-15405.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.63 analysis N-terminal domain plays a critical structural role in maintaining an active configuration of the C-terminal domain, N-hAGT and C-hAGT domains can protect from N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli GWR-109 cells Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expression of N-hAGT and C-hAGT in Escherichia coli XL1-Blue cells. N-hAGT, C-hAGT and full length AGT expressed from the pQE-30 vector in Escherichia coli GWR-109 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.63 C24A mutation of Cys24 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT Homo sapiens
2.1.1.63 C5A mutation of Cys5 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.63 O6-benzylguanine
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.63 Zn2+ N-terminal domain contains a bound zinc atom, and zinc binding confers a mechanistic enhancement to repair activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 by immobilized metal affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine C-terminal domain is totally inactive, the N-terminal domain has a very weak but definite activity that is totally dependent on the refolding being carried out in the presence of zinc ions Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 AGT
-
Homo sapiens
2.1.1.63 C-hAGT
-
Homo sapiens
2.1.1.63 N-hAGT
-
Homo sapiens
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.63 0.00009
-
N-hAGT and C-hAGT complex Homo sapiens O6-benzylguanine
2.1.1.63 0.00022
-
N-hAGT Homo sapiens O6-benzylguanine