EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.63 | analysis | N-terminal domain plays a critical structural role in maintaining an active configuration of the C-terminal domain, N-hAGT and C-hAGT domains can protect from N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli GWR-109 cells | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.63 | expression of N-hAGT and C-hAGT in Escherichia coli XL1-Blue cells. N-hAGT, C-hAGT and full length AGT expressed from the pQE-30 vector in Escherichia coli GWR-109 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.63 | C24A | mutation of Cys24 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT | Homo sapiens |
2.1.1.63 | C5A | mutation of Cys5 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.63 | O6-benzylguanine | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.63 | Zn2+ | N-terminal domain contains a bound zinc atom, and zinc binding confers a mechanistic enhancement to repair activity | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.63 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.63 | by immobilized metal affinity chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.63 | DNA containing 6-O-methylguanine + [protein]-L-cysteine | C-terminal domain is totally inactive, the N-terminal domain has a very weak but definite activity that is totally dependent on the refolding being carried out in the presence of zinc ions | Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.63 | AGT | - |
Homo sapiens |
2.1.1.63 | C-hAGT | - |
Homo sapiens |
2.1.1.63 | N-hAGT | - |
Homo sapiens |
2.1.1.63 | O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.1.1.63 | 0.00009 | - |
N-hAGT and C-hAGT complex | Homo sapiens | O6-benzylguanine | |
2.1.1.63 | 0.00022 | - |
N-hAGT | Homo sapiens | O6-benzylguanine |