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Literature summary extracted from

  • Goda, S.; Kojima, M.; Nishikawa, Y.; Kujo, C.; Kawakami, R.; Kuramitsu, S.; Sakuraba, H.; Hiragi, Y.; Ohshima, T.
    Intersubunit interaction induced by subunit rearrangement is essential for the catalytic activity of the hyperthermophilic glutamate dehydrogenase from Pyrobaculum islandicum (2005), Biochemistry, 44, 15304-15313.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 heating at 90°C the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme Pyrobaculum islandicum
1.4.1.2 Urea at 5 M the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme, urea-induced activation of recombinant GDH is irreversible Pyrobaculum islandicum

Application

EC Number Application Comment Organism
1.4.1.2 additional information subunit rearrangement, i.e., a change in the quaternary structure of the hexameric recombinant GDH, is essential for activation of the enzyme Pyrobaculum islandicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.2 expressed in Escherichia coli (DE3)-codon plus-RIL cells Pyrobaculum islandicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.2 0.018
-
 NAD+ recombinant, urea-activated enzyme Pyrobaculum islandicum
1.4.1.2 0.019
-
 NAD+ native enzyme Pyrobaculum islandicum
1.4.1.2 0.021
-
 NAD+ recombinant, heat-activated enzyme Pyrobaculum islandicum
1.4.1.2 0.16
-
 L-glutamate native enzyme Pyrobaculum islandicum
1.4.1.2 0.16
-
 L-glutamate recombinant, heat-activated enzyme Pyrobaculum islandicum
1.4.1.2 0.18
-
 L-glutamate recombinant, urea-activated enzyme Pyrobaculum islandicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 47000
-
 6 * 47000, SDS-PAGE Pyrobaculum islandicum
1.4.1.2 280000
-
 recombinant enzyme, gel filtration Pyrobaculum islandicum
1.4.1.2 299000
-
 recombinant, heat-activated enzyme, gel filtration Pyrobaculum islandicum
1.4.1.2 337000
-
 recombinant, urea-activated enzyme, gel filtration Pyrobaculum islandicum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Pyrobaculum islandicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2
-
 Pyrobaculum islandicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 3.51
-
 native enzyme Pyrobaculum islandicum
1.4.1.2 3.54
-
 recombinant, urea-activated enzyme Pyrobaculum islandicum
1.4.1.2 3.65
-
 recombinant, heat-activated enzyme Pyrobaculum islandicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pyrobaculum islandicum 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 47000, SDS-PAGE Pyrobaculum islandicum

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 GDH
-
 Pyrobaculum islandicum
1.4.1.2 glutamate dehydrogenase
-
 Pyrobaculum islandicum