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Literature summary extracted from

  • Griffiths, T.A.; Mauk, A.G.; MacGillivray, R.T.
    Recombinant expression and functional characterization of human hephaestin: a multicopper oxidase with ferroxidase activity (2005), Biochemistry, 44, 14725-14731.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.3.1 0.0021
-
 Ferrous ammonium sulfate pH 5.0, 22°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.3.1 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.16.3.1 copper 3.1 atoms per protein molecule Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.16.3.1 121000
-
 x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens
1.16.3.1 129600
-
 x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens
1.16.3.1 143000
-
 x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.1 Homo sapiens
-
 multicopper oxidase hephaestin, with additional ferroxidase activity. Expression in baby hamster kidney cells
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.16.3.1 glycoprotein carbohydrate content of 7.7%, no high mannose oligosaccharides Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.3.1 after expression in baby hamster kidney cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.3.1 apotransferrin + Fe2+ + O2
-
 Homo sapiens diferric transferrin + H2O
-
 ?
1.16.3.1 ferrous ammonium sulfate + O2
-
 Homo sapiens ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.3.1 ? x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.16.3.1 hephaestin
-
 Homo sapiens