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Literature summary extracted from

  • Nazi, I.; Wright, G.D.
    Catalytic mechanism of fungal homoserine transacetylase (2005), Biochemistry, 44, 13560-13566.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.31 mutant enzymes expressed in Saccharomyces cerevisiae Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.31 D209N kcat/Km for acetyl-CoA is 1.45fold lower than wild-type value, kcat/Km for L-homocysteine is 1.6fold higher than wild-type value Schizosaccharomyces pombe
2.3.1.31 D374N kcat/Km for acetyl-CoA is 1.6fold than wild-type value, kcat/Km for L-homocysteine is 1.6fold higher than wild-type value Schizosaccharomyces pombe
2.3.1.31 D403N inactive mutant enzyme Schizosaccharomyces pombe
2.3.1.31 H432A inactive mutant enzyme Schizosaccharomyces pombe
2.3.1.31 S163A inactive mutant enzyme Schizosaccharomyces pombe
2.3.1.31 S163C kcat/Km for acetyl-CoA is 16.7fold than wild-type value, kcat/Km for L-homocysteine is 33fold lower than wild-type value Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.0116
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 0.0199
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 0.0209
-
acetyl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 0.0239
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 0.0441
-
butyryl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 0.0484
-
propionyl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 0.96
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 1.09
-
L-homoserine 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 1.15
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 6.43
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine Schizosaccharomyces pombe the enzyme is a catalyst in the biochemical pathway that metabolizes Asp to Met in fungi CoA + O-acetyl-L-homoserine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine the enzyme is a catalyst in the biochemical pathway that metabolizes Asp to Met in fungi Schizosaccharomyces pombe CoA + O-acetyl-L-homoserine
-
?
2.3.1.31 acetyl-CoA + L-homoserine homoserine transacetylase catalyzes the acetylation of L-homoserine via a covalent acyl-enzyme intermediate through an active-site Ser Schizosaccharomyces pombe CoA + O-acetyl-L-homoserine
-
?
2.3.1.31 butyryl-CoA + L-homoserine
-
Schizosaccharomyces pombe CoA + O-butyryl-L-homoserine
-
?
2.3.1.31 propionyl-CoA + L-homoserine
-
Schizosaccharomyces pombe CoA + O-propionyl-L-homoserine
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.31 0.031 0.51 acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 0.64
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 1.67
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 8.4
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 8.46
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 9.34
-
acetyl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 9.6
-
L-homoserine 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 12.9
-
acetyl-CoA 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 13.4
-
L-homoserine 21°C, pH 8.0, mutant enzyme Schizosaccharomyces pombe
2.3.1.31 16.1
-
propionyl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe
2.3.1.31 47.2
-
butyryl-CoA 21°C, pH 8.0, wild-type enzyme Schizosaccharomyces pombe