EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.2.2 | G179A | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
1.6.2.2 | G179P | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
1.6.2.2 | G179T | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
1.6.2.2 | G179V | mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.001 | - |
ferrocytochrome b5 | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.006 | - |
NADH | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.007 | - |
ferricyanide | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.007 | - |
ferricyanide | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.008 | - |
ferricyanide | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.008 | - |
ferricyanide | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.008 | - |
ferrocytochrome b5 | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.008 | - |
ferricyanide | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.013 | - |
ferrocytochrome b5 | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.025 | - |
NADH | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.043 | - |
ferrocytochrome b5 | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.107 | - |
ferrocytochrome b5 | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.375 | - |
NADPH | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.507 | - |
NADPH | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.595 | - |
NADH | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.662 | - |
NADH | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 0.924 | - |
NADPH | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 1.077 | - |
NADH | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 1.36 | - |
NADPH | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 2.317 | - |
NADPH | mutant G179P, pH 7.0 | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Rattus norvegicus | P20070 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricyanide + NADH | - |
Rattus norvegicus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
1.6.2.2 | 2 ferricyanide + NADPH | - |
Rattus norvegicus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | - |
Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 8 | - |
NADPH | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 10 | - |
ferrocytochrome b5 | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 12 | - |
NADPH | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 12 | - |
ferricyanide | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 17 | - |
NADPH | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 17 | - |
ferrocytochrome b5 | mutant G179V, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 18 | - |
ferrocytochrome b5 | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 33 | - |
NADPH | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 33 | - |
ferricyanide | mutant G179T, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 42 | - |
ferricyanide | mutant G179P, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 48 | - |
NADPH | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 245 | - |
ferrocytochrome b5 | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 400 | - |
ferrocytochrome b5 | wild-type, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 559 | - |
ferricyanide | mutant G179A, pH 7.0 | Rattus norvegicus | |
1.6.2.2 | 800 | - |
ferricyanide | wild-type, pH 7.0 | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | NADH | - |
Rattus norvegicus | |
1.6.2.2 | NADPH | - |
Rattus norvegicus |